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The constitution of the SASP varies among cell types and with the method of senescence induction; nevertheless, there is substantial overlap among SASPs, especially the presence of pro\u2010inflammatory cytokines such as IL\u20101\u03b2, IL\u20101\u03b1, IL\u20106 and IL\u20108. These cytokines are highly conserved among SASPs and are implicated in the development of several cancers. Here, we report that ROCK inhibition by Y\u201027632 reduces levels of IL\u20101\u03b1, IL\u20101\u03b2, IL\u20106 and IL\u20108 secreted by senescent normal and dysplastic oral keratinocytes without affecting the permanent cell growth arrest. The data indicate some inflammatory genes downregulated by Y\u201027632 remain downregulated even after repeated passage in the absence of Y\u201027632. We propose ROCK kinase inhibition as a novel alternative to current strategies to modulate the inflammatory components of the SASP, without compromising the permanent cell growth arrest. This observation potentially has wide clinical applications, given the involvement of senescence in cancer and a wide range of age\u2010related disease. It also suggests care should be exercised when using Y\u201027632 to facilitate cell expansion of primary cells, as its effects on gene expression are not entirely reversible.<\/jats:p>","DOI":"10.1002\/2211-5463.13012","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T14:41:15Z","timestamp":1603464075000},"page":"2740-2749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["ROCK inhibition modulates the senescence\u2010associated secretory phenotype (SASP) in oral keratinocytes"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1858-3091","authenticated-orcid":false,"given":"Sven","family":"Niklander","sequence":"first","affiliation":[{"name":"Unit of Oral and Maxillofacial Medicine, Pathology and Surgery University of Sheffield Sheffield UK"},{"name":"Departamento de Cirugia y Patologia Oral Facultad de Odontologia Universidad Andres Bello 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