{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:00:45Z","timestamp":1747144845878,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T00:00:00Z","timestamp":1605052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:p>Hashimoto's thyroiditis (HT) during pregnancy is usually accompanied by an elevation of thyroid\u2010stimulating hormone and a reduction of serum\u2010free thyroxine during gestation, which may lead to abortion, preterm delivery, and reduced intellectual function of the offspring. Epigenetic alterations may provide important insights into genetic\u2013environmental interactions in HT. Here, we examined global DNA methylation patterns in patients with HT during pregnancy. DNA was extracted from 13 women with HT during pregnancy (HTDP) and eight healthy pregnant women as a control group. Genome\u2010wide methylation was detected with the use of an Illumina Human Methylation 850K Beadchip. A total of 652 differentially methylated positions (DMPs) and 27 differentially methylated regions (DMRs) were identified between the HTDP and control groups. GO analysis revealed that DMPs were significantly enriched in 540 GO terms, which included regulation of the differentiation of keratinocytes, T helper cell differentiation, and alpha\u2010beta T\u2010cell differentiation. Moreover, significant enrichment of KEGG pathways of the DMPs included mucin\u2010type O\u2010glycan biosynthesis, focal adhesion, and the insulin signaling pathway. The GO items associated with DMRs included muscle cell proliferation, response to biotic stimulus, anatomical structure formation involved in morphogenesis, and genes primarily involved in the FoxO signaling pathway. Finally, the DTNA gene was identified as the seed gene of functional epigenetic modules. In summary, the DNA methylation pattern of the HTDP group was distinct from that of the control group, and thus, changes in DNA methylation may influence the development of HT by regulation of the autoimmunity process.<\/jats:p>","DOI":"10.1002\/2211-5463.13018","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T21:02:50Z","timestamp":1603918970000},"page":"2780-2790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genome\u2010wide DNA methylation analysis of Hashimoto's thyroiditis during pregnancy"],"prefix":"10.1002","volume":"10","author":[{"given":"Cai","family":"Wenqian","sequence":"first","affiliation":[{"name":"Eugenic Genetics Laboratory Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital) Tongji Medical College Huazhong University of Science &amp; Technology Wuhan 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