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In addition, miR\u201022 directly targets peroxisome proliferative\u2010activated receptor, Pgc\u20101\u03b1, peroxisome proliferator\u2010activated receptor \u03b1, and sirtuin 1 (Sirt1), which are important factors involved in fatty acid metabolism. Given that miR\u201022 directly targets genes involved in the control of metabolism and obesity, we investigated whether miR\u201022 contributes to metabolic alterations induced by obesity. We observed increased expression of miR\u201022, decreased expression of <jats:italic>Sirt1<\/jats:italic>, and alterations in the expression of adipogenesis\u2010related genes in a mouse model of obesity and a human hepatocyte cell line. We identified that miR\u201022 and the 3\u2032\u2010UTR of <jats:italic>Sirt1<\/jats:italic> are complementary. Mutation of the complementary fragment abolishes the ability of miR\u201022 to regulate the <jats:italic>Sirt1<\/jats:italic> gene. Furthermore, treatment of hepatic steatosis cells with miR\u201022 mimics or inhibitors showed that miR\u201022 can promote hepatic steatosis, and miR\u201022 inhibitors effectively reduced triglyceride levels without affecting cell activity. Finally, we validated that miR\u201022 has similar effects on downstream lipid metabolism\u2010related genes. Our data reveal the pathways and mechanisms through which miR\u201022 regulates lipid metabolism and suggest that miR\u201022 inhibitors may have potential as candidate drugs for NAFLD and obesity.<\/jats:p>","DOI":"10.1002\/2211-5463.13026","type":"journal-article","created":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T03:20:34Z","timestamp":1604719234000},"page":"322-332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["MiR\u201022 modulates the expression of lipogenesis\u2010related genes and promotes hepatic steatosis <i>in\u00a0vitro<\/i>"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6403-9356","authenticated-orcid":false,"given":"Zhuo","family":"Yang","sequence":"first","affiliation":[{"name":"National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Key Laboratory of Trace Element and Nutrition National Health Commission of 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