{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T03:18:43Z","timestamp":1773199123318,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T00:00:00Z","timestamp":1613433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H02585"],"award-info":[{"award-number":["16H02585"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:p>Exosomes are released from a variety of cells to communicate with recipient cells. Exosomes contain microRNAs (miRNAs), which are noncoding RNAs that suppress target genes. Our previous proteomic study (<jats:italic>FEBS Open Bio<\/jats:italic> 2016, <jats:bold>6<\/jats:bold>, 816\u2013826) demonstrated that 3T3\u2010L1 adipocytes secrete exosome components as well as growth factors, inspiring us to investigate what type of miRNA is involved in adipocyte\u2010secreted exosomes and what functions they carry out in recipient cells. Here, we profiled miRNAs in 3T3\u2010L1 adipocyte\u2010secreted exosomes and revealed suppression of muscle differentiation by adipocyte\u2010derived exosomes. Through our microarray analysis, we detected over 300 exosomal miRNAs during adipocyte differentiation. Exosomal miRNAs present during adipocyte differentiation included not only pro\u2010adipogenic miRNAs but also miRNAs associated with muscular dystrophy. Gene ontology analysis predicted that the target genes of miRNAs are associated primarily with transcriptional regulation. To further investigate whether adipocyte\u2010secreted exosomes regulate the expression levels of genes involved in muscle differentiation, we treated cultured myoblasts with adipocyte\u2010derived exosome fractions. Intriguingly, the expression levels of myogenic regulatory factors, Myog and Myf6, and other muscle differentiation markers, myosin heavy\u2010chain 3 and insulin\u2010like growth factor 2, were significantly downregulated in myoblasts treated with adipocyte\u2010derived exosomes. Immature adipocyte\u2010derived exosomes exhibited a stronger suppressive effect than mature adipocyte\u2010derived exosomes. Our results suggest that adipocytes suppress the expression levels of muscle differentiation\u2010associated genes in myoblasts via adipocyte\u2010secreted exosomes containing miRNAs.<\/jats:p>","DOI":"10.1002\/2211-5463.13100","type":"journal-article","created":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T23:49:55Z","timestamp":1612396195000},"page":"768-781","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immature adipocyte\u2010derived exosomes inhibit expression of muscle differentiation markers"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5973-1073","authenticated-orcid":false,"given":"Koichi","family":"Ojima","sequence":"first","affiliation":[{"name":"Muscle Biology Research Unit Division of Animal Products Research National Institute of Livestock and Grassland Science NARO Tsukuba 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