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Seeded assembly of \u03b1\u2010synuclein into filaments is believed to underlie the prion\u2010like spreading of protein inclusions in a number of human neurodegenerative diseases, including Parkinson's disease, dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). We previously determined the atomic structures of \u03b1\u2010synuclein filaments from the putamen of five individuals with MSA. Here, we used filament preparations from three of these brains for the\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    seeded assembly of recombinant human \u03b1\u2010synuclein. We find that the structures of the seeded assemblies differ from those of the seeds, suggesting that additional, as yet unknown, factors play a role in the propagation of the seeds. Identification of these factors will be essential for understanding the prion\u2010like spreading of \u03b1\u2010synuclein proteinopathies.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13110","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T10:07:29Z","timestamp":1612606049000},"page":"999-1013","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":192,"title":["Seeded assembly\n                    <i>in\u00a0vitro<\/i>\n                    does not replicate the structures of \u03b1\u2010synuclein filaments from multiple system atrophy"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2152-1476","authenticated-orcid":false,"given":"Sofia","family":"L\u00f6vestam","sequence":"first","affiliation":[{"name":"MRC Laboratory of Molecular Biology Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1848-1610","authenticated-orcid":false,"given":"Manuel","family":"Schweighauser","sequence":"additional","affiliation":[{"name":"MRC Laboratory of Molecular Biology Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-8284","authenticated-orcid":false,"given":"Tomoyasu","family":"Matsubara","sequence":"additional","affiliation":[{"name":"Department of Neuropathology Tokyo Metropolitan Institute of Gerontology Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Murayama","sequence":"additional","affiliation":[{"name":"Department of Neuropathology Tokyo Metropolitan Institute of Gerontology Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisuke","family":"Tomita","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences The University of Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ando","sequence":"additional","affiliation":[{"name":"Department of Neurology Nagoya University Graduate School of Medicine Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Division of Neurology Sagamihara National Hospital Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Institute for Medical Science of Aging Aichi Medical University Nagakute Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Airi","family":"Tarutani","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences The University of Tokyo Japan"},{"name":"Department of Brain and Neurosciences Tokyo Metropolitan Institute of Medical Science Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Brain and Neurosciences Tokyo Metropolitan Institute of Medical Science Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5214-7886","authenticated-orcid":false,"given":"Michel","family":"Goedert","sequence":"additional","affiliation":[{"name":"MRC Laboratory of Molecular Biology Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-6540","authenticated-orcid":false,"given":"Sjors H. 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