{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T06:32:17Z","timestamp":1722061937205},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T00:00:00Z","timestamp":1622246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>Post\u2010translational modifications, especially reversible phosphorylation, are among the most common mechanisms that regulate protein function and biological processes in <jats:italic>Plasmodium<\/jats:italic> species. Of the <jats:italic>Plasmodium<\/jats:italic> phosphatases, phosphatase of regenerating liver (<jats:italic>Pf<\/jats:italic>PRL) is secreted and is an essential phosphatase. Here, we expressed <jats:italic>Pf<\/jats:italic>PRL in a heterologous expression system, and then purified and characterized its phosphatase activity. We found that Novartis_003209, a previously identified inhibitor, inhibited the <jats:italic>Pf<\/jats:italic>PRL phosphatase activity of recombinant <jats:italic>Pf<\/jats:italic>PRL and blocked <jats:italic>in\u00a0vitro<\/jats:italic> parasite growth in a dose\u2010dependent manner. Further, <jats:italic>in\u00a0silico<\/jats:italic> docking analysis of Novartis_003209 with all four <jats:italic>P.\u00a0falciparum<\/jats:italic> tyrosine phosphatases (PTP) demonstrated that Novartis_003209 is a <jats:italic>Plasmodium<\/jats:italic> PTP inhibitor. Overall, our results identify a scaffold as a potential starting point to design a PTP\u2010specific inhibitor.<\/jats:p>","DOI":"10.1002\/2211-5463.13171","type":"journal-article","created":{"date-parts":[[2021,5,2]],"date-time":"2021-05-02T07:54:59Z","timestamp":1619942099000},"page":"1921-1929","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A <i>Plasmodium\u00a0falciparum<\/i> protein tyrosine phosphatase inhibitor identified from the ChEMBL\u2010NTD database blocks parasite growth"],"prefix":"10.1002","volume":"11","author":[{"given":"Rajan","family":"Pandey","sequence":"first","affiliation":[{"name":"Translational Bioinformatics Group International Centre for Genetic Engineering and Biotechnology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priya","family":"Gupta","sequence":"additional","affiliation":[{"name":"Malaria Biology Group International Centre for Genetic 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