{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:08:40Z","timestamp":1787288920758,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T00:00:00Z","timestamp":1626393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774348"],"award-info":[{"award-number":["81774348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874477"],"award-info":[{"award-number":["81874477"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>Trauma\u2010induced osteonecrosis of the femoral head (TIONFH) is characterized by femoral head collapse accompanied by degenerative changes of the hip. We previously reported that miR\u201093\u20105p expression is abnormally high in patients with TIONFH, but the role of miR\u201093\u20105p in the TIONFH process remains unclear. Herein, we investigated the role of miR\u201093\u20105p in TIONFH in a rabbit model. Bone marrow mesenchymal stem cells (BMSCs) were used for both <jats:italic>in\u00a0vivo<\/jats:italic> and <jats:italic>in\u00a0vitro<\/jats:italic> experiments. A rabbit model of TIONFH was injected with BMSCs transfected with miR\u201093\u20105p inhibitor. In addition, both an miR\u201093\u20105p mimic and negative control were transfected into BMSCs. Expression of miR\u201093\u20105p was significantly increased in the model group compared with control samples. An miR\u201093\u20105p inhibitor induced the expression of bone morphogenetic protein 2 (BMP\u20102) and alkaline phosphatase. Furthermore, expression of osteogenesis\u2010related markers (BMP\u20102, secreted phosphoprotein 1, RUNX family transcription factor 2 and Osterix) was higher in the miR\u201093\u20105p inhibitor group, as revealed by quantitative PCR and western blotting. In addition, <jats:italic>in\u00a0vitro<\/jats:italic> experimentation revealed that an miR\u201093\u20105p mimic decreased BMP\u20102 and TNF receptor superfamily member 11b expression, but increased receptor activator of nuclear factor\u2010kappaB ligand expression. In summary, the miR\u201093\u20105p inhibitor could promote osteogenic differentiation by increasing BMP\u20102 expression during the development of TIONFH. Thus, miR\u201093\u20105p may have potential as a therapeutic target for TIONF treatment.<\/jats:p>","DOI":"10.1002\/2211-5463.13218","type":"journal-article","created":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T12:42:49Z","timestamp":1622983369000},"page":"2152-2165","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Inhibition of miR\u201093\u20105p promotes osteogenic differentiation in a rabbit model of trauma\u2010induced osteonecrosis of the femoral head"],"prefix":"10.1002","volume":"11","author":[{"given":"Ying","family":"Zhang","sequence":"first","affiliation":[{"name":"Medical Center of Hip Luoyang Orthopedic\u2010Traumatological Hospital (Orthopedics Hospital of Henan Province) China"},{"name":"Guangzhou University of Chinese Medicine China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikun","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Guangzhou University 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