{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T09:27:30Z","timestamp":1785058050577,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p><jats:italic>Mycoplasma\u00a0hyorhinis<\/jats:italic> (<jats:italic>M.\u00a0hyorhinis<\/jats:italic>) lacks a cell wall and resists multiple antibiotics. We describe here the striking &gt;\u00a090% inhibitory effect of hemin, a natural inducer of the cytoprotective enzyme heme oxygenase\u20101 (HO\u20101), on <jats:italic>M.\u00a0hyorhinis<\/jats:italic> replication in chronically infected LNCaP prostate cancer cells. The role of HO\u20101 in interrupting <jats:italic>M.\u00a0hyorhinis<\/jats:italic> replication was confirmed by HO\u20101\u2010specific siRNA suppression of hemin\u2010induced HO\u20101 protein expression, which increased intracellular <jats:italic>M.\u00a0hyorhinis<\/jats:italic> DNA levels in LNCaP cells. Proteomic analysis and transmission electron microscopy of hemin\u2010treated cells confirmed the complete absence of <jats:italic>M.\u00a0hyorhinis<\/jats:italic> proteins and intact microorganisms, respectively, strongly supporting these findings. Our study is the first to our knowledge suggesting therapeutic potential for activated HO\u20101 in cellular innate responses against mycoplasma infection.<\/jats:p>","DOI":"10.1002\/2211-5463.13271","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T19:16:07Z","timestamp":1628622967000},"page":"2727-2739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hemin activation abrogates <i>Mycoplasma\u00a0hyorhinis<\/i> replication in chronically infected prostate cancer cells via heme oxygenase\u20101 induction"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1753-7053","authenticated-orcid":false,"given":"Hanxia","family":"Huang","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Dabrazhynetskaya","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-9417","authenticated-orcid":false,"given":"Jacob","family":"Pluznik","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Office of Science and Engineering Laboratories Center for Devices and Radiological Health, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wu","sequence":"additional","affiliation":[{"name":"Office of Science and Engineering Laboratories Center for Devices and Radiological Health, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Chizhikov","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"Buehler","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth M.","family":"Yamada","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5241-5302","authenticated-orcid":false,"given":"Subhash","family":"Dhawan","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration Silver Spring MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2003.00590.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.32607\/20758251-2016-8-2-24-34"},{"key":"e_1_2_10_4_1","unstructured":"Del GiudiceR&GardellaR(1984) Uses and standardization of vertebrate cell cultures. In Tissue Culture Association in\u00a0vitro Monograph Gaithersburg. pp 104."},{"key":"e_1_2_10_5_1","first-page":"306","article-title":"Influence of pasteurella multocida and mycoplasma hyorhinis (PPLO) on the histopathology of field case of swine pneumonia","volume":"52","author":"Roberts ED","year":"1962","journal-title":"Cornell Vet"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.22.10197"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v7.i2.266"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.264"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-10-132"},{"key":"e_1_2_10_10_1","first-page":"e378","article-title":"Mycoplasma hyorhinis: a potential risk factor in gastric cancer progression","volume":"1","author":"Duan H","year":"2014","journal-title":"Cancer Cell Microenviron"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11805-010-0534-8"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006872"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.256"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-11-233"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1094-1156.1998"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(93)90089-R"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00030.2002"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01017-09"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-55-63"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.4252"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1586"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0307172"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.07.021"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903419106"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.4499"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2012.00129"},{"key":"e_1_2_10_27_1","first-page":"53","article-title":"Heme oxygenase\u20101\u2010mediated host cell response inhibits the susceptibility of prostate cancer cells to retroviral infection and retards their proliferation","volume":"14","author":"Zhou ZH","year":"2013","journal-title":"Curr Trends Immunol"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.04.095"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02422-13"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.12.019"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2011.05108.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-198-1_8"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.124.2.1019-1022.1975"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01358-4"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054219"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18764-3"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26071"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.567685"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2223-0"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45539-8"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8091351"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-2191"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1159\/000108302"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140753"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077955"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0040"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-0650"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21647"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-015-9630-8"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.08.018"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.13271","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T03:36:54Z","timestamp":1693280214000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.13271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,9]]},"references-count":49,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1002\/2211-5463.13271"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.13271","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,9]]},"assertion":[{"value":"2021-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}