{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:16:24Z","timestamp":1786976184407,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T00:00:00Z","timestamp":1638662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["20ZR1448700"],"award-info":[{"award-number":["20ZR1448700"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Dental pulp tissue engineering is a promising alternative treatment for pulpitis and periapical periodontitis, and dental pulp stem cells (DPSCs) are considered to be the gold standard for dental seed cell research. Periapical lesions harbor mesenchymal stem cells with the capacity for self\u2010renewal and multilineage differentiation. However, it remains unknown whether these periapical lesion\u2010derived stem cells (PLDSCs) are suitable for dental pulp tissue engineering. To investigate this possibility, PLDSCs and DPSCs were isolated using the tissue outgrowth method and cultured under identical conditions. We then performed\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    experiments to investigate their biological characteristics. Our results indicate that PLDSCs proliferate actively\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    and exhibit similar morphology, immunophenotype and multilineage differentiation ability as DPSCs. Simultaneously, PLDSCs exhibit stronger migrative ability and express more vascular endothelial growth factor and glial cell line\u2010derived neurotrophic factor than DPSCs, and PLDSC\u2010derived conditioned medium was more effective in tube formation assay. The mRNA expression levels of immunomodulatory genes\n                    <jats:italic>HLA\u2010G<\/jats:italic>\n                    ,\n                    <jats:italic>IDO<\/jats:italic>\n                    and\n                    <jats:italic>ICAM\u20101<\/jats:italic>\n                    were also higher in PLDSCs. However, regarding osteo\/odontogenic differentiation, PLDSCs showed weaker alkaline phosphatase staining and lower calcified nodule formation compared to DPSCs, as well as lower expression of\n                    <jats:italic>ALP<\/jats:italic>\n                    ,\n                    <jats:italic>RUNX2<\/jats:italic>\n                    and\n                    <jats:italic>DSPP<\/jats:italic>\n                    , as confirmed by a quantitative RT\u2010PCR. The osteo\/odontogenic protein expression levels of DSPP, RUNX2, DMP1 and SP7 were also higher in DPSCs. The present study demonstrates that PLDSCs demonstrate potential use as seed cells for dental pulp regeneration, especially for achieving enhanced neurovascularization.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13336","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T09:07:33Z","timestamp":1637917653000},"page":"270-284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>In vitro<\/i>\n                    evaluation of periapical lesion\u2010derived stem cells for dental pulp tissue engineering"],"prefix":"10.1002","volume":"12","author":[{"given":"Weiping","family":"Li","sequence":"first","affiliation":[{"name":"Department of Endodontics and Operative Dentistry Shanghai Ninth People\u2019s Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Department of Oral and Maxillofacial Head &amp; Neck Oncology Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengying","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Operative Dentistry Shanghai Ninth People\u2019s Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Operative Dentistry Shanghai Ninth People\u2019s Hospital College of Stomatology Shanghai Jiao Tong 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