{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:00:04Z","timestamp":1793422804614,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T00:00:00Z","timestamp":1641945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100557"],"award-info":[{"award-number":["82100557"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p>Ulcerative colitis is a common inflammatory bowel disease with a complex genetic and immune etiology. Immune infiltration plays a vital role in the development of ulcerative colitis. To explore potential biomarkers for ulcerative colitis and analyze characteristics of immune cell infiltration, we used bioinformatic analyses, including machine learning algorithms, cell type deconvolution methods, and pathway enrichment methods. In this study, we identified 216 differentially expressed mRNAs (DEMs), of which 153 were upregulated, and 63 were downregulated genes. DEMs were mainly enriched in infiltrating neutrophils and regulation of leukocyte migration. Moreover, eight candidate biomarkers, DPP10, MST1L, DPP10\u2010AS1, CEP55, ACSL1, MGP, OLFM4, and SGK1, were identified. Of these candidate biomarkers, MST1L, OLFM4, and DPP10 were then validated in the<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE48958\">GSE48958<\/jats:ext-link>dataset and were predicted to be strongly correlated with infiltrating immune cells of ulcerative colitis. The underlying mechanism of these key genes in the development of colitis was also predicted by gene set variation analysis. To further validate these biomarkers' expression in ulcerative colitis, we determined mRNA levels of<jats:italic>SGK1<\/jats:italic>,<jats:italic>CEP55<\/jats:italic>,<jats:italic>ACSL1<\/jats:italic>,<jats:italic>OLFM4<\/jats:italic>, and<jats:italic>DPP10<\/jats:italic>in lipopolysaccharides (LPS)\u2010stimulated Raw264.7 cells by quantitative reverse transcription\u2010polymerase chain reaction. We also examined<jats:italic>SGK1<\/jats:italic>,<jats:italic>CEP55<\/jats:italic>,<jats:italic>ACSL1<\/jats:italic>,<jats:italic>OLFM4<\/jats:italic>,<jats:italic>DPP10<\/jats:italic>, and<jats:italic>MGP<\/jats:italic>expression in the colon tissues of dextran sodium sulfate\u2010induced colitis mice. Consistent with the predicted computational results, the mRNA levels of these candidate genes were markedly changed in LPS\u2010stimulated Raw264.7 cells and inflamed colon tissues. Hence, our findings indicated that these critical genes may act as diagnostic biomarkers for ulcerative colitis and that differential immune infiltration cells may help illustrate the progression of ulcerative colitis.<\/jats:p>","DOI":"10.1002\/2211-5463.13357","type":"journal-article","created":{"date-parts":[[2021,12,23]],"date-time":"2021-12-23T07:30:29Z","timestamp":1640244629000},"page":"500-515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Integrative computational approach identifies immune\u2010relevant biomarkers in ulcerative colitis"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7873-853X","authenticated-orcid":false,"given":"Tianzhen","family":"He","sequence":"first","affiliation":[{"name":"Institute of Special Environmental Medicine Nantong University 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