{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:16:51Z","timestamp":1786976211385,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81904121"],"award-info":[{"award-number":["81904121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002949","name":"Government of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20191087"],"award-info":[{"award-number":["BK20191087"]}],"id":[{"id":"10.13039\/501100002949","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>\n                    As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte\u2010conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3\u2009days. The primary pericytes were positive for platelet\u2010derived growth factor receptor\u2010\u03b2, \u03b1\u2010smooth muscle actin, neuron\u2010glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte\u2013myofibroblast transition occurred after treatment with transforming growth factor\u2010\u03b21. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    peritoneal fibrosis studies.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13386","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T12:16:01Z","timestamp":1646050561000},"page":"784-797","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation and characterization of peritoneal microvascular pericytes"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2132-0540","authenticated-orcid":false,"given":"Lei","family":"Tang","sequence":"first","affiliation":[{"name":"First Clinic Medical School Nanjing University of Chinese Medicine China"},{"name":"Renal Division Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine &amp; School of Integrated Chinese and Western Medicine Nanjing University of Chinese Medicine China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manshu","family":"Yu","sequence":"additional","affiliation":[{"name":"Renal Division Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Shan","sequence":"additional","affiliation":[{"name":"Renal Division Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Metabolic Diseases in Chinese Medicine Nanjing University of Chinese Medicine 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