{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:19:00Z","timestamp":1768515540397,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T00:00:00Z","timestamp":1651449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>Chaihu, the dried roots of some species of <jats:italic>Bupleurum<\/jats:italic> L., is a famous Chinese herbal medicine for treatment of liver\u2010 and cold\u2010related diseases, in which saikosaponins (SSs) are the major active compounds. Many of the genetic components upstream of SS biosynthetic pathways have been characterized; however, the regulatory mechanisms remain elusive. In this study we identified the APETALA2\/Ethylene Responsive Factor family transcription factor gene <jats:italic>BcERF3<\/jats:italic> from <jats:italic>B.\u2009chinense<\/jats:italic>. The expression of <jats:italic>BcERF3<\/jats:italic> was induced in methyl\u2010jasmonate\u2010treated adventitious root of <jats:italic>B.\u2009chinense<\/jats:italic>; it was also expressed at higher levels in roots than in other tissues (stem, leaf, flower, and tender fruit of early fruiting plants). Transient expression of <jats:italic>BcERF3<\/jats:italic> in the leaves of <jats:italic>Nicotiana\u2009benthamiana<\/jats:italic> resulted in intracellular localization of the protein in the nucleus. It was also demonstrated that the number of SSs was greater in <jats:italic>BcERF3\u2010<\/jats:italic>overexpressing hairy roots of <jats:italic>B.\u2009chinense<\/jats:italic> than in plants treated with empty vector controls. This coincided with upregulation of <jats:italic>\u03b2\u2010AS<\/jats:italic>, which encodes a key enzyme involved with triterpenoid biosynthesis. In conclusion, <jats:italic>BcERF3<\/jats:italic> plays a positive regulatory role in the biosynthesis of SSs.<\/jats:p>","DOI":"10.1002\/2211-5463.13412","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T16:35:13Z","timestamp":1650126913000},"page":"1344-1352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Overexpression of <i>BcERF3<\/i> increases the biosynthesis of saikosaponins in <i>Bupleurum chinense<\/i>"],"prefix":"10.1002","volume":"12","author":[{"given":"Wenjing","family":"Han","sequence":"first","affiliation":[{"name":"Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences &amp; Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education &amp; National Engineering Laboratory for Breeding of Endangered Medicinal Materials)  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences &amp; Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education &amp; National Engineering Laboratory for Breeding of Endangered Medicinal Materials)  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hefang","family":"Wan","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences &amp; Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education &amp; National Engineering Laboratory for Breeding of Endangered Medicinal Materials)  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences &amp; Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education &amp; 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