{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T21:26:42Z","timestamp":1768253202068,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002974"],"award-info":[{"award-number":["82002974"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007452","name":"Wu Jieping Medical Foundation","doi-asserted-by":"publisher","award":["320.6750.2021\u201006\u201030"],"award-info":[{"award-number":["320.6750.2021\u201006\u201030"]}],"id":[{"id":"10.13039\/100007452","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:p>Klinefelter syndrome (KS) is a leading contributor to male infertility and is characterised by complex and diverse clinical features; however, genetic changes in the KS transcriptome remain largely unknown. We therefore used transcriptomic and single\u2010cell RNA sequencing (scRNA\u2010seq) datasets from KS versus normal populations through the Gene Expression Omnibus (GEO) database to identify gene biomarkers associated with the occurrence of KS. We identified a total of 700 differentially expressed genes (DEGs) and completed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), enrichment pathway analysis and protein\u2010protein interaction (PPI) network analysis. A total of four unreported KS\u2010related hub genes (<jats:italic>KIF2C<\/jats:italic>, <jats:italic>MRPS2<\/jats:italic>, <jats:italic>RPS15<\/jats:italic> and <jats:italic>TSFM<\/jats:italic>) were identified. Validation of the single\u2010cell sequencing dataset showed that only <jats:italic>KIF2C<\/jats:italic> and <jats:italic>RPS15<\/jats:italic> were expressed in spermatocytes and that they were differentially expressed in sperm cells. Further construction of the developmental trajectories of these two genes in sperm cells showed that the <jats:italic>KIF2C<\/jats:italic> gene showed an upward trend throughout the differentiation and development of sperm cells. In conclusion, we report here that <jats:italic>KIF2C<\/jats:italic> may be closely related to the differentiation and development of sperm cells in KS patients, which is important for revealing the molecular mechanism of KS and conducting further studies.<\/jats:p>","DOI":"10.1002\/2211-5463.13446","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T16:45:00Z","timestamp":1653669900000},"page":"1465-1474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>KIF2C<\/i> affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by <scp>RNA\u2010Seq<\/scp> and <scp>scRNA\u2010Seq<\/scp> data"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-2337","authenticated-orcid":false,"given":"Haihong","family":"He","sequence":"first","affiliation":[{"name":"Clinical Laboratory Medicine Centre, Shenzhen Hospital Southern Medical 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