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Similar human BCR sequences are frequently found at a significantly higher frequency than that theoretically calculated. Patients infected with SARS\u2010CoV2 and HIV or with autoimmune diseases share very similar BCRs. Therefore, <jats:italic>in silico<\/jats:italic> reconstitution of BCR repertoires and identification of stereotypical BCR sequences related to human pathology have diagnostic potential. Furthermore, monitoring changes of clinically significant BCR sequences and isotype conversion has prognostic potential. For BCR repertoire analysis, peripheral blood (PB) is the most convenient source. However, the optimal human PB volume for <jats:italic>in silico<\/jats:italic> reconstitution of the BCR repertoire has not been studied in detail. Here, we sampled 5, 10, and 20\u2009mL PB from the left arm and 40\u2009mL PB from the right arm of two volunteers, reconstituted <jats:italic>in silico<\/jats:italic> PB BCR repertoires, and compared their composition. In both volunteers, PB sampling over 20\u2009mL resulted in slight increases in functional unique sequences (FUSs) or almost no increase in repertoire diversity. All FUSs with a frequency above 0.08% or 0.03% in the 40\u2009mL PB BCR repertoire were detected even in the 5\u2009mL PB BCR repertoire from each volunteer. FUSs with a higher frequency were more likely to be found in BCR repertoires from reduced PB volume, and those coexisting in two repertoires showed a statistically significant correlation in frequency irrespective of sampled anatomical site. The correlation was more significant in higher\u2010frequency FUSs. These observations support the potential of BCR repertoire analysis for diagnosis.<\/jats:p>","DOI":"10.1002\/2211-5463.13467","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T08:27:18Z","timestamp":1658478438000},"page":"1634-1643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Optimization of peripheral blood volume for <i>in silico<\/i> reconstitution of the human B\u2010cell receptor repertoire"],"prefix":"10.1002","volume":"12","author":[{"given":"Hyunho","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering Seoul National University  South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duck Kyun","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Seoul National University College of Medicine  South Korea"},{"name":"Department of 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