{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:10:47Z","timestamp":1770999047759,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:p>CATSPER2 (Cation channel sperm\u2010associated protein 2) protein, which is part of the calcium CATSPER channel located in the membrane of the flagellar principal piece of the sperm cell, is only expressed in the testis during spermatogenesis. Deletions or mutations in the <jats:italic>Catsper2<\/jats:italic> gene are associated with the deafness\u2010infertility syndrome (DIS) and non\u2010syndromic male infertility. However, the mechanisms by which <jats:italic>Catsper2<\/jats:italic> is regulated are unknown. Here, we report the characterization of the promoter region of murine <jats:italic>Catsper2<\/jats:italic> and the role of CTCF and CREM\u03c4 in its transcription. We report that the promoter region has transcriptional activity in both directions, as determined by observing luciferase activity in mouse Sertoli and GC\u20101 spg transfected cells. WGBS data analysis indicated that a CpG island identified <jats:italic>in silico<\/jats:italic> is non\u2010methylated; Chromatin immunoprecipitation (ChIP)\u2010seq data analysis revealed that histone marks H3K4me3 and H3K36me3 are present in the promoter and body of the <jats:italic>Catsper2<\/jats:italic> gene respectively, indicating that <jats:italic>Catsper2<\/jats:italic> is subject to epigenetic regulation. In addition, the murine <jats:italic>Catsper2<\/jats:italic> core promoter was delimited to a region between \u221254\/+189 relative to the transcription start site (TSS), where three CTCF and one CRE binding site were predicted. The functionality of these sites was determined by mutation of the CTCF sites and deletion of the CRE site. Finally, ChIP assays confirmed that CREM\u03c4 and CTCF bind to the <jats:italic>Catsper2<\/jats:italic> minimal promoter region. This study represents the first functional analysis of the murine <jats:italic>Catsper2<\/jats:italic> promoter region and the mechanisms that regulate its expression.<\/jats:p>","DOI":"10.1002\/2211-5463.13518","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T07:18:45Z","timestamp":1667891925000},"page":"2236-2249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of the promoter region of the murine <i>Catsper2<\/i> gene"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-5199","authenticated-orcid":false,"given":"Andrea del Pilar","family":"Contreras\u2010Marciales","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica y Biolog\u00eda Molecular Centro de Investigaci\u00f3n y Estudios Avanzados del Instituto Polit\u00e9cnico Nacional (CINVESTAV)  Ciudad de M\u00e9xico 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