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Sirtuin 3 (SIRT3) has been reported to promote the degradation of hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1), and thus, we hypothesized that SIRT3 may influence hypoxia\u2010induced apoptosis of spermatogonia. In this study, we overexpressed or inhibited SIRT3 in mouse type B spermatogonia GC\u20102 cells and then subjected the cells to hypoxia or normoxia, before examining hypoxia\u2010responsive gene expression and cell viability. We report that SIRT3 stabilizes hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1) and activates its downstream target gene expression in GC\u20102 cells. We also show that the SIRT3 inhibitor 3\u2010TYP suppresses HIF\u20101\u03b1 target gene expression and alleviates hypoxia\u2010induced apoptosis of GC\u20102 cells. Our study reveals the critical role and underlying mechanisms of SIRT3 in hypoxia\u2010induced apoptosis of mouse type B spermatogonia GC\u20102 cells.<\/jats:p>","DOI":"10.1002\/2211-5463.13523","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T14:19:01Z","timestamp":1668608341000},"page":"154-163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>SIRT3<\/scp> inhibition suppresses hypoxia\u2010inducible factor 1\u03b1 signaling and alleviates hypoxia\u2010induced apoptosis of type B spermatogonia <scp>GC<\/scp>\u20102 cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Zixuan","family":"Wang","sequence":"first","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology Chinese Academy of Sciences  Wuhan 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