{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:36:48Z","timestamp":1785883008964,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["201840273"],"award-info":[{"award-number":["201840273"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>Bone marrow mesenchymal stem cells (BMSCs) are capable of multidirectional differentiation, and engrafted BMSCs can be used to replace damaged chondrocytes for treatment of intervertebral disc disease. However, chondroblast differentiation of implanted BMSCs is inhibited by the anoxic environment of the articular cavity. Here, we found that leptin enhanced the transformation of BMSCs into chondrocytes under hypoxic conditions. BMSCs isolated from mice were cultured in medium supplemented with leptin under hypoxia. The expression of MFN1\/2 and OPA1 were increased only in BMSCs cultured in an anoxic environment. In addition, in hypoxic environments cell energy metabolism relies on glycolysis regulated by leptin, rather than by mitochondrial oxidation. The expression of the de\u2010SUMOylation protease SENP1 was elevated, leading to SIRT3\u2010mediated activation of PGC\u20101\u03b1; these processes were regulated by CREB phosphorylation, and promoted mitochondrial fusion and cell differentiation. The chondrogenic activity of BMSCs isolated from <jats:italic>SIRT3<\/jats:italic>\u2010knockout mice was lower than that of BMSCs isolated from wildtype mice. Implantation of <jats:italic>SIRT3<\/jats:italic>\u2010knockout murine\u2010derived BMSCs did not significantly improve the articular cartilage layer of the disc. In conclusion, the hypoxic microenvironment promoted BMSC differentiation into chondrocytes, whereas osteoblast differentiation was inhibited. SENP1 activated SIRT3 through the deSUMOylation of mitochondria and eliminated the antagonistic effect of SIRT3 acetylation on phosphorylation. When phosphorylation activity of CREB was increased, phosphorylated CREB is then transferred to the nucleus, affecting PGC\u20101\u03b1. This promotes mitochondrial fusion and differentiation of BMSCs. Leptin not only maintains chondrogenic differentiation homeostasis of BMSCs, but also provides energy for differentiation of BMSCs under hypoxic conditions through glycolysis.<\/jats:p>","DOI":"10.1002\/2211-5463.13539","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T13:49:26Z","timestamp":1671544166000},"page":"293-306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Leptin accelerates <scp>BMSC<\/scp> transformation into vertebral epiphyseal plate chondrocytes by activating <scp>SENP1<\/scp>\u2010mediated <scp>deSUMOylation<\/scp> of <scp>SIRT3<\/scp>"],"prefix":"10.1002","volume":"13","author":[{"given":"Xiaomiao","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjian","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zude","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1549-6235","authenticated-orcid":false,"given":"Yi","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31822ef700"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.137836"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.14141082"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3049"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-016-0254-3"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-3"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2017.03.003"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.19-3-180"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.639165"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1876-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.12.4406"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/372425a0"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6093"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13100"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703753200"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07784-7"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.432"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00465"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-1629"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48196-z"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.86"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.05.004"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00390-2"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.2337\/db10-0331"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0388"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601633"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.10.006"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.070169"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501485200"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1093"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205057"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.08.010"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2015.12.001"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws097"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1115172"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13092273"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101443"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2013.165"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-03148-9"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200068"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.13130858"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2020.04.020"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1717128"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.07.023"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13254"},{"key":"e_1_2_11_47_1","first-page":"1056","article-title":"Targeted and sustained Sox9 expression in mouse hypertrophic chondrocytes causes severe and spontaneous osteoarthritis by perturbing cartilage homeostasis","volume":"12","author":"Liang B","year":"2020","journal-title":"Am J Transl Res"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03765-x"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/md12073852"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1802932115"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03487-0"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010157"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.06.008"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13539","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.13539","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13539","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T18:40:44Z","timestamp":1692470444000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.13539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,13]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/2211-5463.13539"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.13539","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,13]]},"assertion":[{"value":"2022-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}