{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T13:59:15Z","timestamp":1747144755513,"version":"3.40.5"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,4,2]],"date-time":"2023-04-02T00:00:00Z","timestamp":1680393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>Corticotropin\u2010releasing factor (CRF) stimulates adrenocorticotropic hormone (ACTH) secretion from the pituitary gland and is an essential regulator of the hypothalamic\u2013pituitary\u2013adrenocortical axis. Isoforms of CRF receptor are known to mediate the effects of urocortin stress ligands on the regulation of stress responses, anxiety, and feeding behavior; however, urocortin stress ligands also influence cell proliferation. In view of the tumor\u2010promoting capacity of prolonged stress, here we investigated (a) the effect of urocortin on cell proliferative signaling via extracellular signal\u2010regulated kinase 1\/2, (b) the expression and cellular distribution of the specific CRF receptor isoforms, and (c) the intracellular localization of phosphorylated ERK1\/2 in HeLa cells. Stimulation of cell proliferation was observed in the presence of 10\u2009n<jats:sc>m<\/jats:sc> urocortin. Our data also suggest that MAP kinase MEK, the transcription factors E2F\u20101 and p53, and PKB\/Akt are involved in this process. These findings may have therapeutic relevance for the targeted treatment of various malignancies.<\/jats:p>","DOI":"10.1002\/2211-5463.13602","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T09:04:19Z","timestamp":1679907859000},"page":"818-832","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Urocortin stimulates the <scp>ERK1<\/scp>\/2 signaling pathway and the proliferation of <scp>HeLa<\/scp> cells via <scp>CRF<\/scp> receptor 1"],"prefix":"10.1002","volume":"13","author":[{"given":"B\u00e1lint","family":"Balogh","sequence":"first","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nika","family":"Vecserny\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"},{"name":"Signal Transduction Research Group J\u00e1nos Szent\u00e1gothai Research Centre  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Stayer\u2010Harci","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"},{"name":"Signal Transduction Research Group J\u00e1nos Szent\u00e1gothai Research Centre  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gergely","family":"Berta","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"},{"name":"Signal Transduction Research Group J\u00e1nos Szent\u00e1gothai Research Centre  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Okt\u00e1via","family":"Tarj\u00e1nyi","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"},{"name":"Signal Transduction Research Group J\u00e1nos Szent\u00e1gothai Research Centre  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2901-8541","authenticated-orcid":false,"suffix":"Jr","given":"Gy\u00f6rgy","family":"S\u00e9t\u00e1l\u00f3","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Central Electron Microscope Laboratory, Medical School University of P\u00e9cs  Hungary"},{"name":"Signal Transduction Research Group J\u00e1nos Szent\u00e1gothai Research Centre  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.10.6517"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.19.8967"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021853"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(01)00046-X"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01631.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.19.8777"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/378287a0"},{"key":"e_1_2_11_9_1","first-page":"4","article-title":"The CRF family of neuropeptides and their receptors \u2013 mediators of the central stress response","volume":"11","author":"Dedic N","year":"2017","journal-title":"Curr Mol Pharmacol"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.186072"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-11-0778"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2011-1361"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890010610"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90688-8"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.5.6590"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2022.111610"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0265-2"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.10094"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2010.06.005"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.08.014"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1023"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-3042.2001.00712.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0689"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1210"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01089.2001"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.08.033"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.04.013"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0565"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01177-4"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051626398"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.06.057"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-11-0048"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2022.01.003"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01594.x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1810"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0255"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607337103"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.88322"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1999.0144"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204869"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.16.2090"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.16.2101"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2010.66"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T17:40:04Z","timestamp":1692466804000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.13602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,2]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/2211-5463.13602"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.13602","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"type":"print","value":"2211-5463"},{"type":"electronic","value":"2211-5463"}],"subject":[],"published":{"date-parts":[[2023,4,2]]},"assertion":[{"value":"2022-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}