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Protein arginine methyltransferase\u20101 (PRMT1) is a key regulator of renal insufficiency. In the present study, we explored the potential involvement of PRMT1 in SI\u2010AKI. A murine model of SI\u2010AKI was induced by cecal ligation and perforation. The expression and localization of PRMT1 and molecules involved in the transforming growth factor (TGF)\u2010\u03b21\/Smad3 and interleukin (IL)\u20106\/signal transducer and activator of transcription 3 (STAT3) signaling pathways were detected in mouse kidney tissues by western blot analysis, immunofluorescence, and immunohistochemistry. The association of PRMT1 with downstream molecules of the TGF\u2010\u03b21\/Smad3 and IL\u20106\/STAT3 signaling pathways was further verified <jats:italic>in vitro<\/jats:italic> in mouse renal tubular epithelial cells. Cecal ligation and perforation caused epithelial\u2013mesenchymal transition, apoptosis, and inflammation in renal tissues, and this was alleviated by inhibition of PRMT1. Inhibition of PRMT1 in SI\u2010AKI mice decreased the expression of TGF\u2010\u03b21 and phosphorylation of Smad3 in the renal cortex, and downregulated the expression of soluble IL\u20106R and phosphorylation of STAT3 in the medulla. Knockdown of PRMT1 in mouse renal tubular epithelial cells restricted the expression of Cox\u20102, E\u2010cadherin, Pro\u2010caspase3, and phosphorylated Smad3 (involved in the TGF\u2010\u03b21\u2010mediated signaling pathway), and also blocked IL\u20106\/soluble IL\u20106R, inducing the expression of Cox\u20102 and phosphorylated\u2010STAT3. In conclusion, our findings suggest that inhibition of PRMT1 mitigates SI\u2010AKI by inactivating the TGF\u2010\u03b21\/Smad3 pathway in the cortex and the IL\u20106\/STAT3 pathway in the medulla. Our findings may aid in the identification of potential therapeutic target molecules for SI\u2010AKI.<\/jats:p>","DOI":"10.1002\/2211-5463.13684","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T07:44:47Z","timestamp":1690875887000},"page":"1859-1873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of <scp>PRMT1<\/scp> alleviates sepsis\u2010induced acute kidney\u00a0injury in mice by blocking the <scp>TGF<\/scp>\u2010\u03b21 and <scp>IL<\/scp>\u20106 trans\u2010signaling pathways"],"prefix":"10.1002","volume":"13","author":[{"given":"Yu","family":"Zhu","sequence":"first","affiliation":[{"name":"Nephrology Department, Shenzhen Hospital University of Chinese Academy of Sciences (Guangming)  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious 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