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In the Shumiya cataract rat, filensin 94\u2009kDa undergoes N\u2010 and C\u2010terminal proteolytic processing to give a transient 50\u2009kDa fragment and a final 38\u2009kDa fragment, just before opacification. To characterize the effects of this processing on filensin function, recombinant proteins representing the two filensin fragments, termed Fil(30\u2013416) and Fil(30\u2013369), respectively, were examined. Fil(30\u2013416) lacks the N\u2010terminal 29 amino acids and the C\u2010terminal 248 amino acids. Fil(30\u2013369) lacks the N\u2010terminal 29 residues and the C\u2010terminal 295 residues. In cell\u2010free assembly characterized by electron microscopy, filensin and Fil(30\u2013416) co\u2010polymerized with phakinin and formed rugged, entangled filaments, whereas Fil(30\u2013369) formed only aggregates. In cultured SW\u201013 and MCF\u20107 cells expressing fluorescent fusion proteins, filensin and Fil(30\u2013416) co\u2010polymerized with phakinin and formed cytoplasmic sinuous filaments with different widths, while Fil(30\u2013369) gave aggregates. Therefore, while truncation of the N\u2010terminal 29 amino acids did not affect filament formation, truncation of the C\u2010terminal 295 but not the 248 residues resulted in failure of filament formation. These results indicate that the tail B region (residues 370\u2013416) of rat filensin is essential for filament formation with phakinin. Truncation of the tail B region by proteolytic processing in the cataract rat lens might interfere with BF formation and thereby contribute to opacification.<\/jats:p>","DOI":"10.1002\/2211-5463.13700","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T15:40:05Z","timestamp":1692891605000},"page":"1990-2004","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of truncations in the N\u2010 and C\u2010terminal domains of filensin on filament formation with phakinin in cell\u2010free conditions and cultured cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Moe","family":"Tashiro","sequence":"first","affiliation":[{"name":"Faculty of Biotechnology and Life Science Sojo University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akari","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Life 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