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Proteolytical cleavage of proNGF to the mature form results in different fragments, whose function and\/or bioactivity is still unclear. The present study was conducted to investigate the distribution of proNGF fragments derived from endogenous cleavage in brain and peripheral tissues of adult rats in the healthy condition and following inflammatory lipopolysaccharide (LPS) challenge. Different anti\u2010proNGF antibodies were tested and the presence of short peptides corresponding to the prodomain sequence (pdNGFpep) was identified. Processing of proNGF was found to be tissue\u2010specific and accumulation of pdNGFpeps was found in inflamed tissues, mainly in testis, intestine and heart, suggesting a possible correlation between organ functions and a response to insults and\/or injury. The bioactivity of pdNGFpep was also demonstrated <jats:italic>in vitro<\/jats:italic> by using primary hippocampal neurons. Our study supports a biological function for the NGF precursor prodomain and indicates that short peptides from residues 1\u201360, differing from the 70\u2013110 sequence, induce apoptosis, thereby opening the way for identification of new molecular targets to study pathological conditions.<\/jats:p>","DOI":"10.1002\/2211-5463.13768","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T05:45:30Z","timestamp":1709358330000},"page":"643-654","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>ProNGF<\/scp> processing in adult rat tissues and bioactivity of <scp>NGF<\/scp> prodomain peptides"],"prefix":"10.1002","volume":"14","author":[{"given":"Marie Anne","family":"Makoudjou","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biology, Department of Biology University of Rome \u201cTor Vergata\u201d  Rome Italy"},{"name":"Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Fico","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Rosso","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Triaca","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR)  Campus A. 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