{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T14:53:03Z","timestamp":1772117583690,"version":"3.50.1"},"reference-count":89,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T00:00:00Z","timestamp":1707609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002790","name":"Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["458021"],"award-info":[{"award-number":["458021"]}],"id":[{"id":"10.13039\/501100002790","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>\n                    Anesthetics have varying physiological effects, but most notably alter ion channel kinetics. Alfaxalone is a rapid induction and washout neuroactive anesthetic, which potentiates \u03b3\u2010aminobutyric acid (GABA)\u2010activated GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor (GABA\n                    <jats:sub>A<\/jats:sub>\n                    \u2010R) currents. This study aims to identify any long\u2010term effects of alfaxalone sedation on pyramidal neuron action potential and GABA\n                    <jats:sub>A<\/jats:sub>\n                    \u2010R properties, to determine if its impact on neuronal function can be reversed in a sufficiently short timeframe to allow for same\u2010day electrophysiological studies in goldfish brain. The goldfish (\n                    <jats:italic>Carassius auratus<\/jats:italic>\n                    ) is an anoxia\u2010tolerant vertebrate and is a useful model to study anoxia tolerance mechanisms. The results show that alfaxalone sedation did not significantly impact action potential properties. Additionally, the acute application of alfaxalone onto naive brain slices caused the potentiation of whole\u2010cell GABA\n                    <jats:sub>A<\/jats:sub>\n                    \u2010R current decay time and area under the curve. Following whole\u2010animal sedation with alfaxalone, a 3\u2010h wash of brain slices in alfaxalone\u2010free saline, with saline exchanged every 30\u2009min, was required to remove any potentiating impact of alfaxalone on GABA\n                    <jats:sub>A<\/jats:sub>\n                    \u2010R whole\u2010cell currents. These results demonstrate that alfaxalone is an effective anesthetic for same\u2010day electrophysiological experiments with goldfish brain slices.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13777","type":"journal-article","created":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T21:35:04Z","timestamp":1707687304000},"page":"555-573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alfaxalone does not have long\u2010term effects on goldfish pyramidal neuron action potential properties or\n                    <scp>\n                      GABA\n                      <sub>A<\/sub>\n                    <\/scp>\n                    receptor currents"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5307-3574","authenticated-orcid":false,"given":"Domenic","family":"Di Stefano","sequence":"first","affiliation":[{"name":"Department of Cell and Systems Biology University 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