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Growing evidence suggests the significance of FNDC3B in tumorigenesis, particularly in cell migration and tumor metastasis. However, its regulatory mechanisms remain elusive. In this study, we employed bioinformatic, gene regulation, and protein\u2010DNA interaction screening to investigate the transcription factors (TFs) involved in regulating FNDC3B. Initially, 338 candidate TFs were selected based on previous chromatin immunoprecipitation (ChIP)\u2010seq experiments available in public domain databases. Through TF knockdown screening and ChIP coupled with Droplet Digital PCR assays, we identified that E2F1 (E2F transcription factor 1) is crucial for the activation of FNDC3B. Overexpression or knockdown of E2F1 significantly impacts the expression of FNDC3B. In conclusion, our study elucidated the mechanistic link between FNDC3B and E2F1. These findings contribute to a better understanding of FNDC3B in tumorigenesis and provide insights into potential therapeutic targets for cancer treatment.<\/jats:p>","DOI":"10.1002\/2211-5463.13783","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T01:23:10Z","timestamp":1708910590000},"page":"687-694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>E2F1<\/scp> promotes cell migration in hepatocellular carcinoma via <scp>FNDC3B<\/scp>"],"prefix":"10.1002","volume":"14","author":[{"given":"Kate","family":"Hua","sequence":"first","affiliation":[{"name":"Cancer Progression Research Center National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen\u2010Tang","family":"Wu","sequence":"additional","affiliation":[{"name":"Cancer Progression Research Center National Yang Ming Chiao Tung University  Taipei 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