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Glutathione transferases are crucial for the establishment and long\u2010term survival of\n                    <jats:italic>T.\u2009solium<\/jats:italic>\n                    ; therefore, we structurally analyzed the 24\u2010kDa glutathione transferase gene (\n                    <jats:italic>Ts24gst<\/jats:italic>\n                    ) of\n                    <jats:italic>T.\u2009solium<\/jats:italic>\n                    and biochemically characterized its product. The gene promoter showed potential binding sites for transcription factors and xenobiotic regulatory elements. The gene consists of a transcription start site, four exons split by three introns, and a polyadenylation site. The gene architecture is conserved in cestodes. Recombinant Ts24GST (rTs24GST) was active and dimeric. Anti\u2010rTs24GST serum showed slight cross\u2010reactivity with human sigma\u2010class GST. A 3D model of Ts24GST enabled identification of putative residues involved in interactions of the G\u2010site with GSH and of the H\u2010site with CDNB and prostaglandin D\n                    <jats:sub>2<\/jats:sub>\n                    . Furthermore, rTs24GST showed optimal activity at 45\u2009\u00b0C and pH 9, as well as high structural stability in a wide range of temperatures and pHs. These results contribute to the better understanding of this parasite and the efforts directed to fight taeniasis\/cysticercosis.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13795","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T23:54:50Z","timestamp":1711065290000},"page":"726-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biochemical characterization and gene structure analysis\u00a0of the 24\u2010k\n                    <scp>D<\/scp>\n                    a glutathione transferase sigma from\n                    <i>Taenia solium<\/i>"],"prefix":"10.1002","volume":"14","author":[{"given":"Ricardo","family":"Miranda\u2010Blancas","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina Universidad Nacional Aut\u00f3noma de M\u00e9xico  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