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Available treatments are limited by their efficacy and side effects. Mesenchymal stem cell\u2010derived extracellular vesicles are presumed as functional equivalents and potential candidates for cell\u2010free therapy. This study reports isolation and characterization of extracellular vesicles from human bone marrow mesenchymal stem cells and evaluates their role in mediating epithelial repair and apoptosis in cultured corneal epithelial cells through scratch assay, PCR, immunofluorescence, and flow cytometry <jats:italic>in vitro<\/jats:italic>. The isolated extracellular vesicles were spherical, &lt;\u2009150\u2009nm in diameter, and characterized as CD9<jats:sup>+<\/jats:sup>, CD63<jats:sup>+<\/jats:sup>, CD81<jats:sup>+<\/jats:sup>, TSG101<jats:sup>+,<\/jats:sup> and Calnexin<jats:sup>\u2212<\/jats:sup>. Further, these vesicles promoted corneal epithelial repair by enhancing proliferation and suppressed apoptosis by regulating the expression of <jats:italic>BAD<\/jats:italic>, <jats:italic>P53<\/jats:italic>, <jats:italic>BCL\u20102<\/jats:italic>, and cleaved CASPASE\u20103<jats:italic>.<\/jats:italic> Thus, our results suggest that BM\u2010MSC\u2010EVs might have the potential to be used for the treatment of injury\u2010induced corneal epithelial defects. Clinical translation of this work would require further investigations.<\/jats:p>","DOI":"10.1002\/2211-5463.13804","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:58:57Z","timestamp":1714438737000},"page":"968-982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Bone marrow mesenchymal stem cell\u2010derived extracellular vesicles promote corneal epithelial repair and suppress apoptosis via modulation of Caspase\u20103 <i>in vitro<\/i>"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8582-5516","authenticated-orcid":false,"given":"Vasudeva","family":"Tati","sequence":"first","affiliation":[{"name":"Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation L V Prasad Eye Institute  Hyderabad India"},{"name":"Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, Centre for Ocular Regeneration L V Prasad Eye Institute  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