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In this study, through\n                    <jats:italic>in silico<\/jats:italic>\n                    simulations, a multi\u2010epitope vaccine against\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    was developed using the mannoprotein antigen (MP88) as a vaccine candidate. Following the retrieval of the MP88 protein sequences, these were used to predict antigenic B\u2010cell and T\u2010cell epitopes via the\n                    <jats:sc>bepipred<\/jats:sc>\n                    tool and the artificial neural network, respectively. Conserved B\u2010cell epitopes AYSTPA, AYSTPAS, PASSNCK, and DSAYPP were identified as the most promising B\u2010cell epitopes. While YMAADQFCL, VSYEEWMNY, and FQQRYTGTF were identified as the best candidates for CD8+\u2009T\u2010cell epitopes; and YARLLSLNA, ISYGTAMAV, and INQTSYARL were identified as the most promising CD4+ T\u2010cell epitopes. The vaccine construct was modeled along with adjuvant and peptide linkers and the\n                    <jats:sc>expasy protparam<\/jats:sc>\n                    tool was used to predict the physiochemical properties. According to this, the construct vaccine was predicted to be antigenic, nontoxic, nonallergenic, soluble, stable, hydrophilic, and thermostable. Furthermore, the three\u2010dimensional structure was also used in docking analyses with Toll\u2010like receptor (TLR4). Finally, the cDNA of vaccine was successfully cloned into the\n                    <jats:italic>E. coli<\/jats:italic>\n                    pET\u201028a (+) expression vector. The results presented here could contribute towards the design of an effective vaccine against\n                    <jats:italic>Cryptococcus neoformans.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13858","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:18Z","timestamp":1721260818000},"page":"1471-1489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Design of an epitope\u2010based peptide vaccine against\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4431-3725","authenticated-orcid":false,"given":"Ibtihal","family":"Omer","sequence":"first","affiliation":[{"name":"Department of Therapeutic Drug Monitoring Laboratory National Center for Kidney Diseases and Surgery  Khartoum 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