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In this study, we characterized two murine embryonic clonal preadipocyte lines, EB5 and EB7, each displaying unique gene marker expression profiles. EB5 cells differentiate into brown adipocytes, whereas EB7 cells into brite (also known as beige) adipocytes. To draw a comprehensive comparison, we contrasted the gene expression patterns, adipogenic capacity, as well as carbohydrate and lipid metabolism of these cells to that of F442A, a well\u2010known white preadipocyte and adipocyte model. We found that commitment to differentiation in both EB5 and EB7 cells can be induced by 3\u2010Isobutyl\u20101\u2010methylxanthine\/dexamethasone (Mix\/Dex) and staurosporine\/dexamethasone (St\/Dex) treatments. Additionally, the administration of rosiglitazone significantly enhances the brown and brite adipocyte phenotypes. Our data also reveal the involvement of a series of genes in the transcriptional cascade guiding adipogenesis, pinpointing GSK3\u03b2 as a critical regulator for both EB5 and EB7 adipogenesis. In a developmental context, we observe that, akin to brown fat progenitors, brite fat progenitors make their appearance in murine development by 11\u201312\u2009days of gestation or potentially earlier. This result contributes to our understanding of adipocyte lineage specification during embryonic development. In conclusion, EB5 and EB7 cell lines are valuable for research into adipocyte biology, providing insights into the differentiation and development of brown and beige adipocytes. Furthermore, they could be useful for the characterization of drugs targeting energy balance for the treatment of obesity and metabolic diseases.<\/jats:p>","DOI":"10.1002\/2211-5463.13861","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T01:36:08Z","timestamp":1720402568000},"page":"1503-1525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gene expression and characterization of clonally derived murine embryonic brown and brite adipocytes"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2377-942X","authenticated-orcid":false,"given":"Cristina","family":"Velez\u2010delValle","sequence":"first","affiliation":[{"name":"Department of Cell Biology Center for Research and Advanced Studies (Cinvestav)  Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia 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