{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T14:58:11Z","timestamp":1772117891058,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T00:00:00Z","timestamp":1725753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n                    This study examined the risk of intrauterine infection associated with radical trachelectomy (RT) in early\u2010stage cervical cancer patients. This procedure preserves fertility but is linked to increased risk of intrauterine infection due to cervical defects during pregnancy. DNA was extracted from the formalin\u2010fixed paraffin\u2010embedded (FFPE) placental specimens of 23 pregnant post\u2010RT patients and 16S rRNA gene sequencing was used for bacterial identification. The prevalence of\n                    <jats:italic>Lactobacillus crispatus<\/jats:italic>\n                    and\n                    <jats:italic>Burkholderia stabilis<\/jats:italic>\n                    was significantly higher in the non\u2010chorioamnionitis group. In contrast, alpha diversity analysis using the PD index showed significantly higher diversity in the chorioamnionitis group (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.04). The demonstrated relationship between chorioamnionitis and microbial diversity affirms the importance of controlling the genital bacterial flora in pregnancies following RT.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13892","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:49:52Z","timestamp":1725842992000},"page":"1825-1836","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbiological investigation of pregnancies following vaginal radical trachelectomy using\n                    <scp>16S rRNA<\/scp>\n                    sequencing of\n                    <scp>FFPE<\/scp>\n                    placental specimens"],"prefix":"10.1002","volume":"14","author":[{"given":"Risa","family":"Tsunematsu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-980X","authenticated-orcid":false,"given":"Tasuku","family":"Mariya","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Umemoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiori","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Arai","sequence":"additional","affiliation":[{"name":"Varinos Inc.  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