{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T14:58:10Z","timestamp":1772117890715,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T00:00:00Z","timestamp":1727049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2023R1A2C1004720"],"award-info":[{"award-number":["2023R1A2C1004720"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002630","name":"Ewha Womans University","doi-asserted-by":"publisher","award":["RP\u2010Grant2022"],"award-info":[{"award-number":["RP\u2010Grant2022"]}],"id":[{"id":"10.13039\/501100002630","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003716","name":"Korea Basic Science Institute","doi-asserted-by":"publisher","award":["2021R1A6C101A442"],"award-info":[{"award-number":["2021R1A6C101A442"]}],"id":[{"id":"10.13039\/501100003716","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    Over the past few decades, VEGF\u2010targeted antiangiogenic therapy for cancers has gained increasing attention. Nevertheless, there are still several limitations such as the potential resistance mechanisms arising in cancer cells against these therapies and their potential adverse effects. These limitations highlight the need for novel anti\u2010angiogenesis molecules and better understanding of the mechanisms of tumor angiogenesis. In the present study, we investigated the antiangiogenic properties of a novel 14\u2010mer antiangiogenic peptide (14\u2010MAP) derived from N\u2010terminal 14\u2009kDa buffalo prolactin and characterized its mode of action. 14\u2010MAP at the picomolar concentration inhibited VEGF\u2010 and bradykinin (an autacoid peptide expressed in vascular tissues in pathophysiology, BK)\u2010stimulated endothelial nitric oxide (eNO) production, cell migration, and proliferation in endothelial cells and vessel development in the chick embryo. Although this peptide inhibited both VEGF\u2010 and BK\u2010dependent angiogenic processes, its action was more pronounced in the latter. Moreover, the interference of 14\u2010MAP with the eNO synthase (eNOS)\u2010cyclic GMP pathway was also identified. A combination of a low dose of Avastin, a widely used drug targeting VEGF\u2010dependent angiogenesis, and 14\u2010MAP significantly reduced tumor size in an\n                    <jats:italic>in vivo<\/jats:italic>\n                    model of human colon cancer. Taken together, our results suggest that 14\u2010MAP, a BK\u2010 and eNOS\u2010dependent antiangiogenic peptide, might be useful for overcoming the limitation of VEGF\u2010targeted antiangiogenic therapy in cancer patients. However, further studies will be required to further characterize its mode of action and therapeutic potential.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13895","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T04:47:49Z","timestamp":1733114869000},"page":"2072-2085","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["14\n                    <scp>K<\/scp>\n                    prolactin derived 14\u2010mer antiangiogenic peptide targets bradykinin\u2010\/nitric oxide\u2010c\n                    <scp>GMP<\/scp>\n                    \u2010dependent angiogenesis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0528-890X","authenticated-orcid":false,"given":"Jaeok","family":"Lee","sequence":"first","affiliation":[{"name":"Pharmaceutical Laboratory, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8233-2273","authenticated-orcid":false,"given":"Pavitra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Vascular Biology Laboratory, AU\u2010KBS Research Centre Anna University, M.I.T. 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Campus  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kambadur","family":"Muralidhar","sequence":"additional","affiliation":[{"name":"Hormone Research Laboratory, Department of Zoology University of Delhi  India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong\u2010Soon","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Life Science Research Korea Basic Science Institute  Daejeon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8706-4810","authenticated-orcid":false,"given":"Hwa Jeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Pharmaceutical Laboratory, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suvro","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Vascular Biology Laboratory, AU\u2010KBS Research Centre Anna University, M.I.T. 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