{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:43:49Z","timestamp":1792813429996,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:p>\n                    Proper glycemic control is crucial for patient management in critical care, including perioperative care, and can influence patient prognosis. Blood glucose concentration determines insulin secretion and sensitivity and affects the intricate balance between the glucose metabolism. Human and other animal studies have demonstrated that perioperative drugs, including volatile anesthetics and intravenous anesthetics, affect glucose\u2010stimulated insulin secretion (GSIS). Dexmedetomidine (DEX) decreases insulin release and affects glucose metabolism; however, the specific mechanism underlying this phenomenon remains largely unknown. Thus, we investigated the effect and mechanism of DEX on insulin secretion using mouse and rat pancreatic \u03b2\u2010cell\u2010derived MIN6 and INS\u20101 cell lines and primary pancreatic \u03b2\u2010cells\/islets extracted from mice. The amount of insulin secreted into the culture medium was determined using an enzyme\u2010linked immunosorbent assay. Cell viability, cytotoxicity, and electrophysiological effects were investigated. Clinically relevant doses of DEX suppressed GSIS in MIN6 cells, INS\u20101 cells, and pancreatic \u03b2\u2010cells\/islets. Furthermore, DEX suppressed insulin secretion facilitated by insulinotropic factors. There was no significant difference in oxygen consumption rate, intracellular ATP levels, or caspase\u20103\/7 activity. Electrophysiological evaluation using the patch\u2010clamp method showed that DEX did not affect ATP\u2010sensitive potassium (K\n                    <jats:sub>ATP<\/jats:sub>\n                    ) channels, voltage\u2010dependent potassium channels, or voltage\u2010gated calcium channels. We demonstrated that clinically relevant doses of DEX significantly suppressed GSIS. These findings suggest that DEX inhibits a signaling pathway via \u03b12\u2010adrenoceptor or insulin vesicle exocytosis, resulting in GSIS suppression. Our results support the hypothesis that DEX suppresses insulin secretion and reveal some underlying mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.13960","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T04:39:44Z","timestamp":1734669584000},"page":"634-646","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dexmedetomidine suppresses glucose\u2010stimulated insulin secretion in pancreatic \u03b2\u2010cells"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7124-6281","authenticated-orcid":false,"given":"Munenori","family":"Kusunoki","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Kansai Medical University  Hirakata Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1110-0827","authenticated-orcid":false,"given":"Kiichi","family":"Hirota","sequence":"additional","affiliation":[{"name":"Department of Pain Clinic Medicine Minamiuonuma City Hospital  Minamiuonuma Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4185-2010","authenticated-orcid":false,"given":"Tomohiro","family":"Shoji","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Mitoyo General Hospital  Kan\u2010onji Kagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeo","family":"Uba","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Kansai Medical University  Hirakata Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3183-4871","authenticated-orcid":false,"given":"Yoshiyuki","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Department of Human Stress Response Science Institute of Biomedical Science, Kansai Medical University  Hirakata Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-5683","authenticated-orcid":false,"given":"Mikio","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Cell Physiology Institute of Biomedical Science, Kansai Medical University  Hirakata Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181948a80"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1213\/01.ane.0000252348.81206.7f"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e3181b8554a"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1498"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181bbcb0d"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8157"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-019-02118-1"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00540-014-1943-2"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ12-0308"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-1-126"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050706"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.130.1.1370150"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.16.1.35"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-016-0273-3"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192796"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27220-8"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-016-1806-9"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55848-7"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-012-2562-9"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115714"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2009.01108.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)46851-2"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205532200"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg389"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200008000-00016"},{"key":"e_1_2_11_27_1","first-page":"72","article-title":"The cell physiology of biphasic insulin secretion","volume":"15","author":"Rorsman P","year":"2000","journal-title":"News Physiol Sci"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16-1166"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2044.1999.00659.x"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb09364.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2013.09.006"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.07.001"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200608132"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-012-2757-0"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8227(11)70010-9"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00401058"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1995.269.5.E897"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/ANA.0b013e318177e5eb"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.13960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T22:00:41Z","timestamp":1743717641000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.13960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/2211-5463.13960"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.13960","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.13960\/v3\/decision1","asserted-by":"object"}]},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,20]]},"assertion":[{"value":"2023-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}