{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:25:59Z","timestamp":1790011559569,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001574"],"award-info":[{"award-number":["82001574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:p>\n                    Heat stress can cause testicular damage and affect male fertility. The gasotransmitter hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) is involved in various physiological and pathophysiological processes, with antioxidant and anti\u2010inflammation effects. The aim of this study was to investigate whether H\n                    <jats:sub>2<\/jats:sub>\n                    S could reduce the testicular damage caused by heat stress. In this study, we constructed a rat heat\u2010stress model and treated it with H\n                    <jats:sub>2<\/jats:sub>\n                    S. Histopathology, high\u2010throughput sequencing, bioinformatics analysis and molecular biology methods were used to investigate whether H\n                    <jats:sub>2<\/jats:sub>\n                    S donor NaHS could alleviate heat stress\u2010induced testicular injury and to explore the underlying mode of action of H\n                    <jats:sub>2<\/jats:sub>\n                    S. Heat stress reduced testis index, sperm quality, sex hormone levels and disturbed testicular structures. High\u2010throughput transcriptome sequencing and Gene Ontology (GO) enrichment analyses showed that genes involved in inflammation were upregulated in the heat stress group compared to the control group. H\n                    <jats:sub>2<\/jats:sub>\n                    S treatment increased testis index, sperm quality and attenuated pathological alternation and hormone disorder in rats exposed to heat stress. Furthermore, H\n                    <jats:sub>2<\/jats:sub>\n                    S administration reversed increased inflammatory factors tumor necrosis factor\u2010\u03b1, interleukin\u20101\u03b2 and interleukin\u20106, and resulted in a significant decrease in malondialdehyde levels and in increased activity of catalase and ratio of reduced\/oxidized glutathione compared to the heat stress group. This study suggests that H\n                    <jats:sub>2<\/jats:sub>\n                    S donor NaHS can effectively restore testicular damage caused by heat stress in rats by inhibiting inflammation and oxidative stress.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70010","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:28:45Z","timestamp":1741739325000},"page":"963-971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hydrogen sulfide repairs testicular damage induced by heat stress in rats"],"prefix":"10.1002","volume":"15","author":[{"given":"Xinyu","family":"Guo","sequence":"first","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"},{"name":"The First Clinical Medical College Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3145-6961","authenticated-orcid":false,"given":"Yupeng","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology General Hospital of Southern Theatre Command  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susu","family":"Guo","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyan","family":"Xie","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqin","family":"Chen","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine The General Hospital of Southern Theater Command  Guangzhou China"},{"name":"The First Clinical Medical College Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9020134"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-22-0200"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00028.2021"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology12081092"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.677110"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2022.2163354"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-45986-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.10.005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6968"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2021.11.019"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13496"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-019-07266-5"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30283"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaaa002"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/02656736.2014.971446"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000453210"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD19303"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14603"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-023-01367-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16428"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37884"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199424000340"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.70010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T06:49:05Z","timestamp":1748846945000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.70010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,5]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/2211-5463.70010"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.70010","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/2211-5463.70010\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.70010\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.70010\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.70010\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/2211-5463.70010\/v2\/review1","asserted-by":"object"}]},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,5]]},"assertion":[{"value":"2024-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}