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This is critical for male gametes, since among the large number of cells present in semen, many may have unnoticeable DNA damage and compromised membrane integrity, among other alterations. In this work, we examined the use of the LensHooke CA0\u2122 device (CA0 chamber) as a promising sperm separation method for ARTs, analyzing both normozoospermic and teratozoospermic samples. Additionally, we compared fertilization rates with IVF and ICSI procedures using Teratozoospermic samples. As reference for comparison, we used the current standard for sperm selection, density gradient centrifugation. Using CA0 chambers and DGC, we obtained comparable sperm recovery numbers, membrane potential (in Normozoospermic samples) and motility parameters. The progesterone\u2010induced intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    increase was only slightly greater in sperm selected with DGC compared to CA0 chambers. Finally, no differences were observed in IVF and ICSI fertilization rates between Tz sperm separated with DGC and CA0 chambers. Overall, we conclude that the quantity and quality of sperm selected with CA0 chambers is comparable to that obtained with DGC, without compromising reproducibility. Importantly, CA0 chambers offer key practical and methodological advantages, resulting in a faster, simpler and more affordable sperm selection method.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70073","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T04:39:14Z","timestamp":1752554354000},"page":"1471-1484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional parameters of spermatozoa obtained by a new selection device"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7375-9531","authenticated-orcid":false,"given":"Julio C.","family":"Ch\u00e1vez","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda (IBT) Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Cuernavaca Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9271-2205","authenticated-orcid":false,"given":"Paulina","family":"Torres","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda (IBT) Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Cuernavaca Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5868-1541","authenticated-orcid":false,"given":"Gabriela","family":"Carrasquel\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"CITMER, Reproductive Medicine  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda G.","family":"Figueroa\u2010M\u00e9ndez","sequence":"additional","affiliation":[{"name":"CITMER, Reproductive Medicine  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0844-2582","authenticated-orcid":false,"given":"Diana","family":"Flores","sequence":"additional","affiliation":[{"name":"CITMER, Reproductive Medicine  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Villar","sequence":"additional","affiliation":[{"name":"CITMER, Reproductive Medicine  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israel","family":"Maldonado","sequence":"additional","affiliation":[{"name":"CITMER, Reproductive Medicine  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela B.","family":"Trevino","sequence":"additional","affiliation":[{"name":"Science Department, School of Pure and Applied Science Florida SouthWestern (FSW) State College  Fort Myers FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7281-0534","authenticated-orcid":false,"given":"Claudia","family":"Trevi\u00f1o","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda (IBT) Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM)  Cuernavaca Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"WHO(2023)Infertility Prevalence Estimates 1990\u20132021."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xfre.2022.11.013"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12978-021-01293-7"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12913-022-08485-2"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11113-023-09765-3"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-108"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.29252\/ijrm.16.7.463"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4471-3317-9_7"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)60622-2"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/14.3.759"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-121"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-1208.192070"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2013.09.005"},{"key":"e_1_2_11_15_1","volume-title":"In Vitro Fertilization, Embryo Transfer and Early Pregnancy. 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