{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:12:47Z","timestamp":1791216767126,"version":"4.1.0"},"reference-count":77,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    Relatively little is known about the microbial variations within the human appendix niche. To overcome this knowledge gap, we employed endoscopic retrograde appendicitis treatment (ERAT) technology to collect microbial samples from the appendix lumen, followed by shotgun metagenomic sequencing on participants with acute appendicitis without antibiotic treatment. Compared to the cecum and terminal ileum, the appendix had a higher abundance at the genus level of\n                    <jats:italic>Sphingobium<\/jats:italic>\n                    ,\n                    <jats:italic>Leptotrichia<\/jats:italic>\n                    and\n                    <jats:italic>Oribacterium<\/jats:italic>\n                    , as well as a significant increase in species\u2010level abundance of oral bacteria, including\n                    <jats:italic>Streptococcus sanguinis<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus australis<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    sp. A12,\n                    <jats:italic>Leptotrichia<\/jats:italic>\n                    sp. oral taxon 215,\n                    <jats:italic>Veillonella dispar<\/jats:italic>\n                    ,\n                    <jats:italic>Veillonella infantium<\/jats:italic>\n                    and\n                    <jats:italic>Oribacterium sinus<\/jats:italic>\n                    . Pearson correlation analysis showed that bacterial species abundant in the appendix, such as\n                    <jats:italic>Acinetobacter johnsonii<\/jats:italic>\n                    ,\n                    <jats:italic>Sphingobium yanoikuyae<\/jats:italic>\n                    and\n                    <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    , had negative correlations with the top five most abundant Gene Ontology (GO) categories (molecular function, biological process and cellular component). Conversely, species underrepresented in the appendix, including\n                    <jats:italic>Mogibacterium diversum<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus sanguinis<\/jats:italic>\n                    ,\n                    <jats:italic>Megasphaera micronuciformis<\/jats:italic>\n                    and\n                    <jats:italic>Actinomyces graevenitzii<\/jats:italic>\n                    , had significant positive correlations with these GO categories. Our results show that ERAT technology can be used to improve sampling and microbiome profiling in the appendix. Furthermore, this in\u2010depth microbial characterization could inform clinicians during antibiotic prescription. However, further large sample size studies are required to validate these results.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70105","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T04:55:44Z","timestamp":1755924944000},"page":"2001-2020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7440-2027","authenticated-orcid":false,"given":"Huimin","family":"Ma","sequence":"first","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery The First Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhu","family":"Feng","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Xie","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixiang","family":"Kong","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Feng","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Cuiying Biomedical Research Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Hospital &amp; Clinical Medical School Lanzhou University  China"},{"name":"Department of Gastroenterology The Second Hospital of Lanzhou University  China"},{"name":"Digestive Endoscopy Center The Second Hospital of Lanzhou University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.20502"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v30.i28.3386"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1027769"},{"key":"e_1_2_11_5_1","first-page":"881","article-title":"A randomized trial comparing antibiotics with appendectomy for appendicitis. 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