{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:53:10Z","timestamp":1790286790780,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["09120012010107"],"award-info":[{"award-number":["09120012010107"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["NWO 3DNI p21 305"],"award-info":[{"award-number":["NWO 3DNI p21 305"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["NWO ENPPS.LIFT.019.030"],"award-info":[{"award-number":["NWO ENPPS.LIFT.019.030"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["Roadmap NLBI 184.036.012"],"award-info":[{"award-number":["Roadmap NLBI 184.036.012"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["754425"],"award-info":[{"award-number":["754425"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    Advanced microscopy techniques, combined with a diverse set of fluorescent probes, provide valuable tools for uncovering insights into biological systems and addressing fundamental research questions. However, the need to develop and use genetic tags and probe markers presents notable challenges. Coherent Raman scattering microscopy offers a label\u2010free alternative, enabling live\u2010cell imaging of cellular structures without the need for labeling. Leveraging the benefits of Raman microscopy, we aim to analyze the pancreas in living zebrafish larvae and to evaluate chemical changes in pancreatic exocrine and endocrine compartments following exocrine damage. Here, we present a protocol for Raman\u2010based label\u2010free microscopic analysis of the pancreas in living zebrafish larvae. Using forward stimulated Raman scattering (F\u2010SRS) and epi coherent anti\u2010Stokes Raman scattering (E\u2010CARS), zebrafish pancreatic structures were analyzed and validated. Vibrational Raman spectra between 450 and 3100\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    were acquired to identify chemical structural features within pancreatic regions. Raman imaging allows discrimination of distinct structures at 2850 and 2934\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    in pancreatic exocrine and endocrine regions, which could mainly correspond to lipids and proteins, respectively. Exocrine damage causes a significant reduction in both the number and size of exocrine granules. Moreover, changes at 2934\u2009cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    suggested chemical alterations in both exocrine and beta\u2010cell regions. In conclusion, SRS and CARS provide a powerful, label\u2010free approach for live\u2010cell imaging and chemical analysis in islet biology. Given the relative straightforward applicability in the pancreas, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical research fields.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70163","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T11:15:34Z","timestamp":1764069334000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Raman\u2010based label\u2010free microscopic analysis of the pancreas in living zebrafish larvae"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1458-9402","authenticated-orcid":false,"given":"Noura","family":"Faraj","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University Medical Center Groningen, University of Groningen  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9213-3211","authenticated-orcid":false,"given":"Eline M. F.","family":"de Lange","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University Medical Center Groningen, University of Groningen  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaas A.","family":"Sjollema","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University Medical Center Groningen, University of Groningen  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5105-5915","authenticated-orcid":false,"given":"Ben N. G.","family":"Giepmans","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University Medical Center Groningen, University of Groningen  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"802","article-title":"Green fluorescent protein as a marker for gene expression","volume":"263","author":"Chalfie M","year":"1994","journal-title":"Science (1979)"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1126\/science.1124618","article-title":"The fluorescent toolbox for assessing protein location and function","volume":"312","author":"Giepmans B","year":"2006","journal-title":"Science"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1002\/cyto.a.22295","article-title":"Fluorescence microscopy\u2010a historical and technical perspective","volume":"83","author":"Renz 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