{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T15:10:41Z","timestamp":1778944241450,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004099","name":"Ligue Contre le Cancer","doi-asserted-by":"publisher","award":["RS22\/75\u2010100"],"award-info":[{"award-number":["RS22\/75\u2010100"]}],"id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>\n                    Colorectal cancer (CRC) incidence and mortality continue to rise globally and new prognostic biomarkers are required for the development of targeted therapies. Several studies have suggested that tissue kallikrein\u2010related peptidases (KLKs), including KLK7, contribute to tumorigenesis. We previously demonstrated KLK7's tumor\u2010promoting role both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , but its role in CRC metastasis remains unclear. Here, using the Cancer Genome Atlas (TCGA), we confirmed that KLK7 expression is upregulated in advanced stages of CRC and its association with shorter progression\u2010free survival (PFS) of patients. To further understand the role of KLK7 in CRC metastasis, we assessed its expression in ascites from CRC patients with peritoneal metastasis (PM), investigated cell behavior following KLK7 overexpression, and examined its role in metastasis using a mouse model. High KLK7 levels were found in malignant ascites, but not in benign ascites. In xenograft models, KLK7\u2010overexpressing cells increased PM and exhibited higher Peritoneal Cancer Index (PCI) scores compared to controls.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , KLK7 overexpression in HT29\u2010D4 human colon cancer cells significantly enhanced cell proliferation, colony formation, migration, spheroid formation, and adhesion to extracellular matrix proteins. Additionally, KLK7 overexpression altered cell morphology, upregulated moesin (MSN) and integrin subunits, suggesting cytoskeletal remodeling and matrix interactions. Taken together, these findings suggest that KLK7 is a driver of CRC progression and could serve as a potential prognostic marker for aggressive forms of CRC.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70171","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:46:12Z","timestamp":1764783972000},"page":"1000-1019","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>KLK7<\/scp>\n                    overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells"],"prefix":"10.1002","volume":"16","author":[{"given":"Yosr Z.","family":"Haffani","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9 CNRS UMR8263\u2010INSERM U1345, Institut de Biologie Paris Seine  France"},{"name":"Laboratory of Physiopathology, Alimentation &amp; Biomolecules PAB, LR17ES03, Higher Institute of Biotechnology\u2010Sidi Thabet University of Mannouba  Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Dreyer","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, Department of Obstetrics and Gynecology Technische Universit\u00e4t M\u00fcnchen  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meriem","family":"Naim","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9 CNRS UMR8263\u2010INSERM U1345, Institut de Biologie Paris Seine  France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rea Lo","family":"Dico","sequence":"additional","affiliation":[{"name":"National Cancer Institute Regina Elena (IRCCS)  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia A.","family":"Ignatenko","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine The University of Arizona  Tucson AZ 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