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In this context, the selection of subclones that acquire enhanced proliferative capacity and resistance to pharmacological treatments represents a necessary step in tumour progression. In the presence of DNA damage, efficient signalling and lesion removal are crucial in preventing the accumulation of genomic mutations. In Nucleotide Excision Repair (NER) pathways, the loss of interaction between DDB2 and PCNA (referred to as DDB2\n                    <jats:sup>PCNA\u2010<\/jats:sup>\n                    ) results in genomic instability, delayed damage removal and activation of the epithelial\u2010to\u2010mesenchymal transition (EMT) transcriptional program. In this study, we demonstrate that human cellular clones, derived from UV\u2010damaged cells expressing DDB2\n                    <jats:sup>PCNA\u2010<\/jats:sup>\n                    , exhibit behaviours typically associated with tumour heterogeneity. Specifically, these clones show anchorage\u2010independent growth, differences in cell cycle regulation, frequency of atypical mitoses, expression of oncogenes and tumour suppressors and resistance to cisplatin treatment. Taken together, these findings highlight the critical role of DDB2\u2010PCNA interaction in maintaining genome stability and preventing cellular transformation.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70195","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:32:19Z","timestamp":1776400339000},"page":"1181-1196","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anchorage\u2010independent and faster growth in clonal population from\n                    <scp>UV<\/scp>\n                    \u2010irradiated\n                    <scp>NER<\/scp>\n                    \u2010deficient cells"],"prefix":"10.1002","volume":"16","author":[{"given":"Paola","family":"Perucca","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Molecolare Universit\u00e0 degli studi di Pavia  Pavia 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