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Recent studies have revealed the involvement of TARM1 in immune\u2010mediated diseases. Despite its biological importance, research tools for elucidating human TARM1 function have been limited. Here, we generated monoclonal antibodies (mAbs) against human TARM1 by a yeast display platform combined with a human single\u2010chain variable fragment (scFv) library. Two scFv clones were isolated and converted into IgG1 antibodies, both of which bound recombinant TARM1 with high affinity and cell\u2010surface TARM1. Importantly, these antibodies induced activation signals into Jurkat NFAT\u2010GFP reporter cells expressing TARM1\u2013CD28\u20134\u20101BB\u2013CD3\u03b6 chimera, indicating agonistic activity. These mAbs provide valuable tools for dissecting TARM1\u2010mediated function and represent a potential approach for therapeutic strategies in inflammatory diseases and cancer.<\/jats:p>","DOI":"10.1002\/2211-5463.70216","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T03:21:13Z","timestamp":1775791273000},"page":"1358-1369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of human monoclonal antibodies against\n                    <scp>TARM1<\/scp>\n                    by yeast display"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7542-8819","authenticated-orcid":false,"given":"Rikio","family":"Yabe","sequence":"first","affiliation":[{"name":"Cancer Immunology Project Tokyo Metropolitan Institute of Medical Science  Setagaya 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