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We examined iron homeostasis in mouse gastrocnemius muscle following 14\u2010day casting\u2010induced immobilization. Muscle mass declined by ~\u200925%, accompanied by increased nonheme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated, suggesting disruption of IRP\/IRE feedback control. Additionally, levels of 4\u2010hydroxynonenal, a marker of lipid peroxidation, were elevated without compensatory responses from SLC7A11\/xCT or GPx4. These findings indicate that short\u2010term immobilization disrupts iron regulatory mechanisms and induces oxidative stress exceeding antioxidant capacity. Iron dysregulation and lipid peroxidation may contribute to the early pathogenesis of disuse\u2010induced muscle atrophy and represent potential therapeutic targets.<\/jats:p>","DOI":"10.1002\/2211-5463.70223","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T16:45:00Z","timestamp":1773938700000},"page":"1501-1508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short\u2010term immobilization"],"prefix":"10.1002","volume":"16","author":[{"given":"Haruka","family":"Yokogawa","sequence":"first","affiliation":[{"name":"Laboratory of Exercise Nutrition, Department of Nutrition University of Shiga Prefecture  Hikone 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