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In the gut commensal\n                    <jats:italic>Bacteroides thetaiotaomicron<\/jats:italic>\n                    (\n                    <jats:italic>B. theta<\/jats:italic>\n                    ), DUF4465 containing proteins act as high\u2010affinity B\n                    <jats:sub>12<\/jats:sub>\n                    \u2013binding proteins that scavenge this cofactor to ensure bacterial survival. Such B\n                    <jats:sub>12<\/jats:sub>\n                    capture is essential for bacteria that have lost the ability to synthesize B\n                    <jats:sub>12<\/jats:sub>\n                    <jats:italic>de novo<\/jats:italic>\n                    . This raises the question of whether B\n                    <jats:sub>12<\/jats:sub>\n                    \u2010binding is ubiquitous across this family of proteins. Here, we show that B\n                    <jats:sub>12<\/jats:sub>\n                    \u2010binding is a recurrent function of eight distantly related members of the DUF4465 family. It is reasonable to conclude that B\n                    <jats:sub>12<\/jats:sub>\n                    \u2010binding is a common function of most DUF4465 proteins. These results establish DUF4465 as a structurally conserved family of augmented \u03b2\u2010jellyroll B\n                    <jats:sub>12<\/jats:sub>\n                    \u2010binding proteins with widespread roles in microbial competition for this essential cofactor.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"box\" position=\"anchor\">\n                      <jats:caption>\n                        <jats:title>Impact statement<\/jats:title>\n                      <\/jats:caption>\n                      <jats:p>\n                        DUF4465 defines a widespread, structurally conserved bacterial cobalamin\u2010binding domain and provides a promising scaffold for protein\u2010based B\n                        <jats:sub>12<\/jats:sub>\n                        capture and purification.\n                      <\/jats:p>\n                    <\/jats:boxed-text>\n                  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