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Addressing this unmet therapeutic need requires non\u2010invasive strategies for generating patient\u2010specific RPE cells. This study reports the successful generation and initial characterization of RPE cells derived from urine\u2010derived induced pluripotent stem cells (u\u2010iPSC\u2010RPE). Urine\u2010derived stem cells (USCs) were isolated from healthy individuals and comprehensively characterized, confirming strong expression of renal progenitor makers and mesenchymal stem cell markers, while lacking standard hematopoietic markers. USCs were reprogrammed into iPSCs using the integration\u2010free Sendai virus expressing the Yamanaka factors. The reprogrammed u\u2010iPSC clones displayed characteristic pluripotency marker expression and demonstrated clearance of the Sendai virus by later passages. Subsequently, these u\u2010iPSCs were efficiently differentiated into RPE cells, exhibiting characteristic hexagonal morphology and pigmentation which was confirmed by the expression of key RPE\u2010specific proteins. Our findings demonstrate the feasibility and reliability of generating patient\u2010specific u\u2010iPSC\u2010RPE cells from readily accessible USCs providing a foundation for future studies to investigate their functional potential for retinal disease modeling and therapeutic applications for AMD.<\/jats:p>","DOI":"10.1002\/2211-5463.70246","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:38:57Z","timestamp":1777070337000},"page":"1691-1707","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Derivation and characterization of retinal pigment epithelium from urine\u2010derived\n                    <scp>iPSCs<\/scp>"],"prefix":"10.1002","volume":"16","author":[{"given":"Daniella","family":"Beiner","sequence":"first","affiliation":[{"name":"Wake Forest Institute for Regeneration Medicine Wake Forest School of Medicine  Winston\u2010Salem NC 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