{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:59:38Z","timestamp":1790881178296,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:p>\n                    Tumor microenvironment plays a pivotal role in regulating the biological properties of cancer cells. Interstitial fluid hydrostatic pressure (HP) generally increases in malignant solid tumors. While our recent work has demonstrated that elevated HP stabilizes HIF\u20101\u03b1 expression in cancer cells, here we further investigate whether elevated HP promotes cancer cells to acquire stemness features. Our results showed that exposure to 50\u2009mmHg HP for 48\u2009h did not significantly change the survival or morphology of human breast cancer cells. However, 50\u2009mmHg HP stimulation significantly increased aldehyde dehydrogenase (ALDH) activity and upregulated HIF\u20101\u03b1 and stemness markers (CD44 and SSEA\u20101) in MCF\u20107 and BT\u2010474 but not in MDA\u2010MB\u2010453 breast cancer cell lines. Correspondingly, \u03b2\u2010catenin was significantly enhanced in MCF\u20107 and BT\u2010474 breast cancer cell lines under 50\u2009mmHg HP stimulation and was negatively expressed in MDA\u2010MB\u2010453 breast cancer cells. Hypoxia (1% O\n                    <jats:sub>2<\/jats:sub>\n                    ) exposure for 48\u2009h also significantly enhanced the expression of \u03b2\u2010catenin only in MCF\u20107 cells and BT\u2010474 breast cancer cell lines, but increased HIF\u20101\u03b1 expression and ALDH activity in all breast cancer cell lines tested, including the \u03b2\u2010catenin\u2010deficient MDA\u2010MB\u2010453 cells. Therefore, we conclude that elevated HP in malignant tumors may facilitate the acquisition of \u2018stemness\u2019 characteristics of cancer cells by enhancing HIF\u20101\u03b1 expression via \u03b2\u2010catenin.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70267","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T16:40:52Z","timestamp":1780332052000},"page":"1992-2002","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hydrostatic pressure activates\n                    <scp>HIF<\/scp>\n                    \u20101\u03b1 via \u03b2\u2010catenin to promote stemness in breast cancer cells"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8245-8290","authenticated-orcid":false,"given":"Da","family":"Zhai","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology Northern Jiangsu People's Hospital  Yangzhou 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