{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:18:29Z","timestamp":1788283109184,"version":"build-2803163510"},"reference-count":82,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014940","name":"Chinese Polar Environment Comprehensive Investigation and Assessment Programmes","doi-asserted-by":"publisher","award":["2021YFA1100401"],"award-info":[{"award-number":["2021YFA1100401"]}],"id":[{"id":"10.13039\/501100014940","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014940","name":"Chinese Polar Environment Comprehensive Investigation and Assessment Programmes","doi-asserted-by":"publisher","award":["2024YFA1108200"],"award-info":[{"award-number":["2024YFA1108200"]}],"id":[{"id":"10.13039\/501100014940","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170745"],"award-info":[{"award-number":["32170745"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570651"],"award-info":[{"award-number":["32570651"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>The Warburg effect has long suggested that oxidative phosphorylation (OXPHOS) is dispensable for tumor growth. However, recent studies have shown that the mitochondrial RNA polymerase inhibitors IMT1 and IMT1b, which impair OXPHOS, are potent anticancer agents. Here, we demonstrate that ionomycin, a selective ionophore known to modulate mitochondrial homeostasis, similarly inhibits mitochondrial gene expression across cancer cell lines. Specifically, gene expression and nascent RNA profiling revealed a global downregulation of mitochondrial gene transcription in Jurkat T, THP\u20101, HeLa, and NCI\u2010H441 cells. Thus, we conclude that ionomycin suppressed mitochondrial gene transcription, impaired OXPHOS, and thereby inhibited cancer cell proliferation and growth, providing a novel insight into the function of ionomycin.<\/jats:p>","DOI":"10.1002\/2211-5463.70297","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:41:26Z","timestamp":1788280886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription"],"prefix":"10.1002","author":[{"given":"Lishen","family":"Wang","sequence":"first","affiliation":[{"name":"Biomedical Sciences College &amp; Shandong Medicinal Biotechnology Centre Shandong First Medical University &amp; Shandong Academy of Medical Sciences, NHC Key Laboratory of Biotechnology Drugs, Shandong Key Laboratory of Genetic Engineering and Synthetic Biology  Jinan China"},{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingqiang","family":"Deng","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"Department of Basic Research Guangzhou National Laboratory  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"He","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diqin","family":"Lai","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengyong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Tang","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanman","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghui","family":"Yin","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0389-4233","authenticated-orcid":false,"given":"Xichen","family":"Bao","sequence":"additional","affiliation":[{"name":"Center for Cell Lineage Atlas Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong\u2010Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  China"},{"name":"Guangzhou Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010018\u20100124\u20101"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.02.011"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.09.025"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.15.1899"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10234"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3412"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200608073"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2011.04.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207105"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"5892","DOI":"10.1016\/S0021-9258(17)34550-7","article-title":"Characterization of ionomycin as a calcium ionophore","volume":"253","author":"Liu C","year":"1978","journal-title":"J Biol Chem"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/313318a0"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201012\u20100210"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02795"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.11553"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202945200"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10435"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1600963"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600465"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2012.04.002"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.12.021"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.036"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010cellbio\u2010092910\u2010154237"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742\u20104658.2007.05686.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201017\u20103070"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u201003048\u2010z"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201007023\u20108"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010023\u201005682\u20107"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201006444\u20109"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09652"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162228"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4595"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.59717\/j.xinn\u2010life.2025.100143"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot087361"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201067018\u20107"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20104176\u20107_10"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr260"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3791\/51998"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1602033"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.267898"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.12.018"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260819"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201709111"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adu5646"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.4161\/trns.2.3.16172"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1130"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp903"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601845"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh339"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262\u2010025\u201003949\u2010w"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.12.017"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2019.108309"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01571\u201007"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2016.02.004"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.02.002"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adr3498"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010024\u201001926\u20103"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010021\u201000415\u20100"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00025.2007"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3038"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010024\u201002119\u20103"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2012.10.004"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201023\u20101006"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2020.100143"},{"key":"e_1_2_10_66_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1042\/bj3480607","article-title":"Evidence that metformin exerts its anti\u2010diabetic effects through inhibition of complex 1 of the mitochondrial respiratory chain","volume":"348","author":"Owen MR","year":"2000","journal-title":"Biochem J"},{"key":"e_1_2_10_67_1","first-page":"108","article-title":"Cooperative patient care in sphincter training for anal incontinence","volume":"43","author":"Flick M","year":"1989","journal-title":"Krankenpflege (Frankf)"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1207912109"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.06.019"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.02.034"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.10.015"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010019\u20100557\u2010x"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u20100155\u20105"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.10.023"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15754"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258944"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081386"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm4039"},{"key":"e_1_2_10_79_1","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.4049\/jimmunol.135.6.3669","article-title":"T\u00a0cell activation: differences in the signals required for IL 2 production by nonactivated and activated T\u00a0cells","volume":"135","author":"Manger B","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_10_80_1","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.4049\/jimmunol.143.4.1283","article-title":"Mechanisms of T\u00a0cell activation by the calcium ionophore ionomycin","volume":"143","author":"Chatila T","year":"1989","journal-title":"J Immunol"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.05.016"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.7.4476"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010immunol\u2010042617\u2010053019"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.70297","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.70297","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.70297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:41:28Z","timestamp":1788280888000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.70297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":82,"alternative-id":["10.1002\/2211-5463.70297"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.70297","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"2026-01-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2211-5463.70297"}}