{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:59:42Z","timestamp":1790881182751,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:p>\n                    The study of proteins that sense and respond to light reveals how nature has harnessed light for function while also mitigating its damaging effects through finely tuned molecular mechanisms. Time\u2010resolved optical spectroscopy and time\u2010resolved X\u2010ray solution scattering and diffraction techniques are well\u2010positioned to probe functional transitions triggered by light in photoactive proteins. However, integrating both fields presents technical and conceptual difficulties for many researchers. This\n                    <jats:italic>FEBS Open Bio<\/jats:italic>\n                    \u2018In the Limelight\u2019 special issue, Dynamic Photobiology, showcases five research protocols that provide comprehensive and practical guidance for designing and analyzing time\u2010resolved experiments on proteins. The protocols cover studies performed in both solution and crystalline environments using time\u2010resolved femtosecond Vis\/IR spectroscopy, time\u2010resolved X\u2010ray solution scattering, and time\u2010resolved serial crystallography at synchrotron and XFEL facilities. These detailed research protocols aim to ease collaboration across fields, acquaint early\u2010career researchers with essential concepts, and offer a comprehensive overview for established researchers.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70303","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:04:06Z","timestamp":1790877846000},"page":"1844-1846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic photobiology: A collection of experimental protocols for time\u2010resolved structural studies on light\u2010activated proteins"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1286-9929","authenticated-orcid":false,"given":"Sofia M","family":"Kapetanaki","sequence":"first","affiliation":[{"name":"MAX IV Laboratory Lund University  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