{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T18:06:28Z","timestamp":1786125988487,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>Prostate cancer (PCa) is the most commonly diagnosed noncutaneous malignancy in men and a leading cause of cancer\u2010related death worldwide. Its clinical heterogeneity, ranging from indolent to aggressive disease, presents major diagnostic and therapeutic challenges. However, prostate\u2010specific antigen (PSA), the current standard biomarker, lacks sufficient sensitivity and specificity, resulting in both overdiagnosis and missed cases. Therefore, more accurate noninvasive biomarkers are urgently needed. In this study, we isolated plasma\u2010derived extracellular vesicles (EVs) using a wheat germ agglutinin (WGA)\u2010conjugated magnetic bead method and identified differentially expressed transcripts between PCa patient and healthy control (HC) EVs. Candidate biomarkers were selected through weighted gene co\u2010expression network analysis (WGCNA) and Random Forest modeling, followed by validation using RT\u2010qPCR in independent cohorts including PCa, benign prostatic hyperplasia (BPH), and HC samples. We identified three EV\u2010derived RNAs (NM_024955, NR_047469, and NR_002564), which were significantly dysregulated in PCa compared with both HC and BPH. Combined analysis demonstrated high diagnostic performance, outperforming PSA and notably NM_024955 expression showed a strong correlation, indicating potential relevance to disease severity. These findings suggest that lectin\u2010based EV isolation coupled with RNA profiling provides a robust and scalable platform for PCa diagnosis, offering promising noninvasive biomarkers to complement or improve current PSA\u2010based screening.<\/jats:p>","DOI":"10.1002\/2211-5463.70305","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:39:58Z","timestamp":1786124398000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Accurate and noninvasive prostate cancer detection using plasma\u2010derived extracellular vesicle\n                    <scp>RNA<\/scp>"],"prefix":"10.1002","author":[{"given":"Hanping","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Urology Wujin Hospital Affiliated with Jiangsu University  Changzhou China"},{"name":"Department of Urology The Wujin Clinical College of Xuzhou Medical University  Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology Wujin Hospital Affiliated with Jiangsu University  Changzhou China"},{"name":"Department of Urology The Wujin Clinical College of Xuzhou Medical University  Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2546-2597","authenticated-orcid":false,"given":"Wei","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Urology Wujin Hospital Affiliated with Jiangsu University  Changzhou China"},{"name":"Department of Urology The Wujin Clinical College of Xuzhou Medical University  Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray F","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"S1976","DOI":"10.4103\/jpbs.jpbs_1742_24","article-title":"AUA guidelines for screening of prostate cancer 2023","volume":"17","author":"Agrawal R","year":"2025","journal-title":"J Pharm Bioallied Sci"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1056\/NEJMoa0810084","article-title":"Screening and prostate\u2010cancer mortality in a randomized European study","volume":"360","author":"Schroder FH","year":"2009","journal-title":"N Engl J Med"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1056\/NEJM200105033441806","article-title":"Clinical practice. 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