{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T09:14:06Z","timestamp":1783588446878,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    A critical limitation of DNA vaccines and other therapeutics is transfection\n                    <jats:italic>in vivo<\/jats:italic>\n                    to produce the encoded antigens or therapeutic proteins. Cutaneous suction\u2010based methods have demonstrated effectiveness in many animal models and have been successfully applied in human clinical trials, but have not been extended to mouse models, where numerous disease models, transgenic strains, and murine\u2010specific reagents exist. Here, we establish and optimize methods for cutaneous suction\u2010mediated DNA transfection in mice. By adapting a smaller cup diameter and smaller injection volume, the challenges of skin hyperelasticity and decreased skin thickness can be effectively addressed. Thus, we demonstrate that vaccinating mice with the GLS\u20105310 SARS\u2010CoV\u20102 DNA vaccine using this method yields high levels of binding antibody and T\u2010cell responses. Additionally, suction following injection of a novel pVAX1\u2010based expression vector yielded systemic levels of a SEAP transgene. We conclude that suction\u2010mediated delivery of nucleic acid\u2010based therapies and vaccines can be a valuable tool for the study of preclinical mouse models.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70306","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:27:50Z","timestamp":1783585670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Delivery of\n                    <scp>DNA<\/scp>\n                    \u2010encoded vaccines and proteins in mice using cutaneous suction\u2010mediated transfection"],"prefix":"10.1002","author":[{"given":"Emran O.","family":"Lallow","sequence":"first","affiliation":[{"name":"GeneOne Life Science  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Brandtjen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9306-0318","authenticated-orcid":false,"given":"Yaxin","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Gulley","sequence":"additional","affiliation":[{"name":"GeneOne Life Science  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Osorio","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Child Health Institute of New Jersey Rutgers Medical School  New Brunswick New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagar","family":"Kudchodkar","sequence":"additional","affiliation":[{"name":"GeneOne Life Science  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandita C.","family":"Jhumur","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine C.","family":"Roberts","sequence":"additional","affiliation":[{"name":"GeneOne Life Science  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa K.","family":"Denzin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Child Health Institute of New Jersey Rutgers Medical School  New Brunswick New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David I.","family":"Shreiber","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biju","family":"Parekkadan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Rutgers University\u2010New Brunswick  New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel N.","family":"Maslow","sequence":"additional","affiliation":[{"name":"GeneOne Life Science  Seoul South Korea"},{"name":"Department of Medicine Morristown Medical Center  Morristown New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1332939","article-title":"The next\u2010generation DNA vaccine platforms and delivery systems: advances, challenges and prospects","volume":"15","author":"Lu B","year":"2024","journal-title":"Front Immunol"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.actbio.2018.08.033","article-title":"Engineering DNA vaccines against infectious diseases","volume":"80","author":"Lee J","year":"2018","journal-title":"Acta Biomater"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines11061016","article-title":"DNA vaccines for epidemic preparedness: SARS\u2010CoV\u20102 and beyond","volume":"11","author":"Maslow JN","year":"2023","journal-title":"Vaccines (Basel)"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/S0140-6736(22)00151-9","article-title":"Efficacy, safety, and immunogenicity of the DNA SARS\u2010CoV\u20102 vaccine (ZyCoV\u2010D): the interim efficacy results of a phase 3, randomised, double\u2010blind, placebo\u2010controlled study in India","volume":"399","author":"Khobragade A","year":"2022","journal-title":"Lancet"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1038\/mt.2009.27","article-title":"Tolerability of two sequential electroporation treatments using MedPulser DNA delivery system (DDS) in healthy adults","volume":"17","author":"Wallace M","year":"2009","journal-title":"Mol Ther"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2021.107871","article-title":"Cell death due to electroporation\u2013a review","volume":"141","author":"Napotnik TB","year":"2021","journal-title":"Bioelectrochemistry"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","DOI":"10.3389\/fviro.2022.891540","article-title":"Immune responses of a novel bi\u2010cistronic SARS\u2010CoV\u20102 DNA vaccine following intradermal immunization with suction delivery","volume":"2","author":"Jeong M","year":"2022","journal-title":"Front Virol\u2010Lausanne"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ijid.2022.12.037","article-title":"Safety and immunogenicity of the bi\u2010cistronic GLS\u20105310 COVID\u201019 DNA vaccine delivered with the GeneDerm suction device","volume":"128","author":"Kim WJ","year":"2023","journal-title":"Int J Infect Dis"},{"key":"e_1_2_11_10_1","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.1016\/j.vaccine.2023.06.013","article-title":"Immune response enhancement with GLS\u20105310 DNA primary vaccine against SARS\u2010CoV\u20102 followed by administration of an mRNA vaccine heterologous boost","volume":"41","author":"Kim WJ","year":"2023","journal-title":"Vaccine"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abj0611","article-title":"Novel suction\u2010based in vivo cutaneous DNA transfection platform","volume":"7","author":"Lallow EO","year":"2021","journal-title":"Sci Adv"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1007\/s00204-023-03446-y","article-title":"Preclinical safety assessment of the suction\u2010assisted intradermal injection of the SARS\u2010CoV\u20102 DNA vaccine candidate pGO\u20101002 in white rabbit","volume":"97","author":"Oh S","year":"2023","journal-title":"Arch Toxicol"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0041319","article-title":"In vivo site\u2010specific transfection of naked plasmid DNA and siRNAs in mice by using a tissue suction device","volume":"7","author":"Shimizu K","year":"2012","journal-title":"PLoS One"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0228203","article-title":"Tissue suction\u2010mediated gene transfer to the beating heart in mice","volume":"15","author":"Taniguchi Y","year":"2020","journal-title":"PLoS One"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2023.105623","article-title":"A cell culture system to model pharmacokinetics using adjustable\u2010volume perfused mixing chambers","volume":"91","author":"Erickson P","year":"2023","journal-title":"Toxicol In Vitro"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","DOI":"10.1002\/adtp.202400055","article-title":"Tissue tension and strain as indicators of suction\u2010mediated cutaneous DNA transfection: a parametric study","volume":"7","author":"Jhumur NC","year":"2024","journal-title":"Adv Ther"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","DOI":"10.3389\/fddev.2023.1095181","article-title":"Molecular distribution in intradermal injection for transfer and delivery of therapeutics","volume":"3","author":"Lallow EO","year":"2023","journal-title":"Front Drug 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vivo rat skin absorption studies","volume":"23","author":"Ravenzwaay B","year":"2004","journal-title":"Hum Exp Toxicol"},{"key":"e_1_2_11_22_1","doi-asserted-by":"crossref","first-page":"15885","DOI":"10.1038\/s41598-017-15830-7","article-title":"Allometric scaling of skin thickness, elasticity, viscoelasticity to mass for micro\u2010medical device translation: from mice, rats, rabbits, pigs to humans","volume":"7","author":"Wei JCJ","year":"2017","journal-title":"Sci Rep"},{"key":"e_1_2_11_23_1","first-page":"170","article-title":"Mouse models of human disease: an evolutionary perspective","volume":"2016","author":"Perlman RL","year":"2016","journal-title":"Evol Med Public Health"},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature01262","article-title":"Initial sequencing and comparative analysis of the mouse genome","volume":"420","author":"Mouse Genome Sequencing, 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evidence for systemic, transient ER stress during endotoxemia","volume":"34","author":"Hiramatsu N","year":"2006","journal-title":"Nucleic Acids Res"}],"container-title":["FEBS Open 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History"}},{"value":"2026-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2211-5463.70306"}}