{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T12:34:00Z","timestamp":1786624440481,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    Myeloid sarcoma (MS) is a rare extramedullary manifestation of acute myeloid leukemia (AML). Conventional two\u2010dimensional cultures fail to replicate the constraints of the MS microenvironment. We demonstrated that 5% gelatin methacrylate (GelMA) hydrogels provide a tunable three\u2010dimensional matrix for\n                    <jats:italic>in vitro<\/jats:italic>\n                    modeling of MS. GelMA hydrogels exhibited tissue\u2010like stiffness and oxygen diffusion properties, supporting the formation of viable spheroids from myeloid leukemia cell lines. The 3D context induced G1 cell cycle arrest and apoptotic progression, reflecting dormancy at extramedullary sites. RNA sequencing revealed transcriptional reprogramming in GelMA\u2010embedded spheroids, with an enrichment in ECM remodeling and metabolic pathways. Furthermore, transcriptomic profiles from 3D\u2010cultured cells aligned with patient\u2010derived MS samples, validating the model's relevance to MS. Thus, we present a GelMA\u2010based system which provides a clinically relevant platform for investigating MS biology and screening potential therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70321","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T11:59:52Z","timestamp":1786622392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>GelMA<\/scp>\n                    \u2010based\n                    <scp>3D<\/scp>\n                    spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0867-3245","authenticated-orcid":false,"given":"Nicolas","family":"Germain","sequence":"first","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut de Recherche Contre le Cancer de Lille, UMR9020 \u2013 UMR\u2010S 1277 \u2013 Canther 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