{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T11:40:01Z","timestamp":1786621201080,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>Cell\u2010free RNA (cfRNA) is emerging as a supplemental approach for liquid biopsy (LBx). Given that lymphoid cells and plasma cells express substantial quantities of immunoglobulin (Ig) and T\u2010cell receptor (TCR) RNA, we investigated the utility of using cfRNA for detecting B\u2010cell and T\u2010cell clonality in LBxs. Using next\u2010generation sequencing (NGS) of cfRNA, we clonotyped Igs and TCRs in LBx samples from patients with B\u2010cell neoplasm, T\u2010cell neoplasm, myeloid neoplasm and solid tumors alongside cancer\u2010free individuals. To establish a clonality cutoff, we clonotyped tissue RNA from patients with confirmed clonal B\u2010 or T\u2010cell and from polyclonal. Clonotype\u2010na\u00efve testing of LBx samples demonstrated B\u2010cell clonality in 36% of B\u2010cell neoplasms, 7% of normal, 8% of T\u2010cell lymphomas, 13% of myeloid neoplasms, and 14% of solid tumors. T\u2010cell clonality using TCR beta or gamma demonstrated T\u2010cell clonality in 22% of T\u2010cell neoplasms, 3% of normal, 5% of myeloid neoplasms, 7% of solid tumors and 3% of B\u2010cell neoplasms. This confirms that Clonotype\u2010na\u00efve cfRNA testing in LBx is a reliable approach for detecting B\u2010 and T\u2010cell clonality.<\/jats:p>","DOI":"10.1002\/2211-5463.70323","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T11:22:20Z","timestamp":1786620140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Using cell\u2010free\n                    <scp>RNA<\/scp>\n                    to identify B\u2010 and T\u2010cell clonality for diagnosis and monitoring of B\u2010 and T\u2010cell neoplasms"],"prefix":"10.1002","author":[{"given":"Adam","family":"Albitar","sequence":"first","affiliation":[{"name":"Genomic Testing Cooperative  Lake Forest CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Agersborg","sequence":"additional","affiliation":[{"name":"Genomic Testing Cooperative  Lake Forest CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Charifa","sequence":"additional","affiliation":[{"name":"Genomic Testing Cooperative  Lake Forest CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Phull","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noa","family":"Biran","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Vesole","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh","family":"Parmar","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Pecora","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0456-5618","authenticated-orcid":false,"given":"Andrew","family":"Ip","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Goy","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Siegel","sequence":"additional","affiliation":[{"name":"John Theurer Cancer Center, Hackensack University Medical Center  NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0050-2030","authenticated-orcid":false,"given":"Maher","family":"Albitar","sequence":"additional","affiliation":[{"name":"Genomic Testing Cooperative  Lake Forest CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2023.06.011"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25338"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020004056"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2019.1627877"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061505"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12071539"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2018.05.008"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.206177"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470\u20102045(15)70106\u20103"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102015\u201003\u2010635169"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201011310\u20100"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12865\u2010014\u20100029\u20100"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e16261"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlb.2024.100270"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2024.286023"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.70076"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100568\u20102"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.70323","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.70323","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.70323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T11:22:21Z","timestamp":1786620141000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.70323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":18,"alternative-id":["10.1002\/2211-5463.70323"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.70323","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]},"assertion":[{"value":"2026-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2211-5463.70323"}}