{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:26:50Z","timestamp":1787128010946,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013589","name":"Research England","doi-asserted-by":"publisher","award":["Expanding Excellence in England \u201cgrant named\u201d"],"award-info":[{"award-number":["Expanding Excellence in England \u201cgrant named\u201d"]}],"id":[{"id":"10.13039\/501100013589","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    Cellulosomes are efficient enzymatic nanomachines which have arisen for the degradation of cellulosic biomass. They are found abundantly in soil\u2010dwelling microbes and bacteria which thrive in the stomachs of ruminant mammals. Two protein domains, cohesins and dockerins, characterise cellulosomes. These domains interact with each other to form, in many cases, enormous complexes with as many as 160 individual proteins. However, genome annotation of\n                    <jats:italic>Cellulosilyticum lentocellum DSM 5427<\/jats:italic>\n                    revealed a single cohesin domain (encoded by Clole_2599) and a single dockerin domain (Clole_2598). Therefore, we recombinantly expressed ClcC and ClcD and found they form a (predicted ~\u2009104\u2009kDa) heterodimeric complex. We show that this complex formation enhances cellulase activity approximately 2\u2010fold on insoluble microcrystalline cellulose and 1.25\u2010fold on soluble carboxymethyl cellulose. Moreover, we identified two additional candidate interacting partners for ClcC, one of which appears to bind via a non\u2010canonical interface. These findings suggest that cellulosomal principles can operate in highly reduced cohesin\u2010dockerin systems.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70330","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:59:18Z","timestamp":1787126358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A minimal cellulosome\u2010like system in\n                    <i>Cellulosilyticum lentocellum<\/i>"],"prefix":"10.1002","author":[{"given":"John","family":"Allan","sequence":"first","affiliation":[{"name":"Department of Applied Sciences, Health and Life Sciences Ellison Building, Northumbria University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9569-4461","authenticated-orcid":false,"given":"Amias","family":"Alstrom\u2010Moore","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences, Health and Life Sciences Ellison Building, Northumbria University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W","family":"Black","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences, Health and Life Sciences Ellison Building, Northumbria University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/nrmicro.2016.164","article-title":"Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides","volume":"15","author":"Artzi L","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"13791","DOI":"10.1073\/pnas.0813093106","article-title":"On the remarkable mechanostability of scaffoldins and the mechanical clamp motif","volume":"106","author":"Valbuena A","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/s41598-017-00919-w","article-title":"Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin\u2010dockerin complexes","volume":"7","author":"Bule P","year":"2017","journal-title":"Sci Rep"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1638551","article-title":"Structural and functional insights into cellulosomes: masters of plant cell wall degradation","volume":"16","author":"Lindi\u010d N","year":"2025","journal-title":"Front Microbiol"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.sbi.2018.03.012","article-title":"Cellulosome assembly: paradigms are meant to be broken!","volume":"49","author":"Bule P","year":"2018","journal-title":"Curr Opin Struct Biol"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00443-20","article-title":"Minimalistic cellulosome of the butanologenic bacterium Clostridium saccharoperbutylacetonicum","volume":"11","author":"Hevroni BL","year":"2020","journal-title":"MBio"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0723-2020(86)80074-1","article-title":"Clostridium lentocellum sp. nov., a cellulolytic species from river sediment containing paper\u2010mill waste","volume":"8","author":"Murray WD","year":"1986","journal-title":"Syst Appl Microbiol"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1128\/JB.00239-11","article-title":"Complete genome sequence of the cellulose\u2010degrading bacterium Cellulosilyticum lentocellum","volume":"193","author":"Miller DA","year":"2011","journal-title":"J Bacteriol"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1099\/ijs.0.014712-0","article-title":"Cellulosilyticum ruminicola gen. Nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov","volume":"60","author":"Cai S","year":"2010","journal-title":"Int J Syst Evol Microbiol"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1046\/j.1472-765X.2003.01397.x","article-title":"Fermentation of cellulose to acetic acid by Clostridium lentocellum SG6: induction of sporulation and effect of buffering agent on acetic acid production","volume":"37","author":"Tammali R","year":"2003","journal-title":"Lett Appl Microbiol"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"11179","DOI":"10.1039\/C9RA01158G","article-title":"Lignocellulosic hydrogen production using dark fermentation by Clostridium lentocellum strain Cel10 newly isolated from Ailuropoda melanoleuca excrement","volume":"9","author":"Zhang L","year":"2019","journal-title":"RSC 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