{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:21:30Z","timestamp":1787552490271,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101825"],"award-info":[{"award-number":["82101825"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    Bacillus Calmette\u2013Gu\u00e9rin (BCG) vaccination has well\u2010documented off\u2010target effects in early life, but the underlying molecular mechanisms remain incompletely understood. This study investigated whether BCG reprograms neonatal myeloid\u2010derived suppressor cells (MDSCs) through oxidative phosphorylation (OXPHOS). After neonatal mice received a subcutaneous injection of BCG or PBS, the effects of BCG exposure on splenic monocytic (M\u2010) and polymorphonuclear (PMN\u2010) MDSCs were assessed. BCG vaccination significantly increased the expression of OXPHOS\u2010related genes (\n                    <jats:italic>Ndufab1, Sdhd, Uqcrfs1, Cox4i<\/jats:italic>\n                    ) and mitochondrial oxidative activity in both MDSC subsets. Seahorse analysis revealed a higher oxygen consumption rate in BCG\u2010exposed PMN\u2010MDSCs. Functional assays showed that BCG impaired MDSC\u2010mediated T\u2010cell suppression, with oligomycin treatment partially able to restore this suppressive capacity. Together, these findings indicate that BCG\u2010induced OXPHOS potentiation contributes to the loss of MDSC immunosuppressive function in neonatal mice, providing a molecular mechanism for the non\u2010specific immunomodulatory effects of BCG vaccination early in life.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70333","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:00:08Z","timestamp":1787551208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>BCG<\/scp>\n                    vaccination potentiates oxidative phosphorylation in neonatal myeloid\u2010derived suppressor cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1669-3003","authenticated-orcid":false,"given":"Yingying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  China"},{"name":"Tianjin Institute of Immunology, Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education, Department of Immunology, School of Basic Medical Sciences Tianjin Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Immunology, Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education, Department of Immunology, School of Basic Medical Sciences Tianjin Medical University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1093\/ije\/dyh392","article-title":"BCG vaccination scar associated with better childhood survival in Guinea\u2010Bissau","volume":"34","author":"Roth A","year":"2005","journal-title":"Int J Epidemiol"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1016\/S0264-410X(03)00181-6","article-title":"BCG scar and positive tuberculin reaction associated with reduced child mortality in West Africa. 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