{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:07:45Z","timestamp":1789992465911,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004541","name":"Ministry of Education, India","doi-asserted-by":"publisher","award":["MoE STARS\/STARS1\/385"],"award-info":[{"award-number":["MoE STARS\/STARS1\/385"]}],"id":[{"id":"10.13039\/501100004541","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["SRG\/2019\/001360"],"award-info":[{"award-number":["SRG\/2019\/001360"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["F.30\u2010431\/2018\u2010BSR"],"award-info":[{"award-number":["F.30\u2010431\/2018\u2010BSR"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>\n                    Arginyltransferase 1 (Ate1), a conserved eukaryotic enzyme, has recently been structurally characterised; identifying a conserved N\u2010terminal domain, a central GNAT fold and a variable C\u2010terminal domain of unknown function. Here, using budding yeast as an experimental model, we established that the C\u2010terminal domain is indispensable for Ate1 stability, its enzymatic activity and Ate1\u2010mediated cell death. Furthermore, we found that the co\u2010expression of separated N\u2010terminal and C\u2010terminal halves of Ate1 did not reconstitute enzyme activity\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Also, we observed that the mutation of conserved residues involved in cofactor binding and active site formation within the N\u2010terminal half of Ate1 abolishes the enzyme function. Moreover, we showed that mouse Ate1, when heterogeneously expressed in yeast, was catalytically active but not lethal. These findings underscore the structural interdependence of Ate1 domains and their integrity in maintaining enzyme catalytic activity and stability, providing deeper insights into the enzyme structure\u2013function relationship.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.70336","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:14:57Z","timestamp":1789989297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The C\u2010terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9147-7588","authenticated-orcid":false,"given":"Vikas Kumar","family":"Yadav","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics and Biochemistry, Department of Botany, Institute of Science Banaras Hindu University  Varanasi UP India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0814-3266","authenticated-orcid":false,"given":"Shweta","family":"Shweta","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics and Biochemistry, Department of Botany, Institute of Science Banaras Hindu University  Varanasi UP India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics and Biochemistry, Department of Botany, Institute of Science Banaras Hindu University  Varanasi UP India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9319-1069","authenticated-orcid":false,"given":"Vikash","family":"Maurya","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics and Biochemistry, Department of Botany, Institute of Science Banaras Hindu University  Varanasi UP 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